Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Chemical modification of enzymes to improve biocatalytic performance

P Giri, AD Pagar, MD Patil, H Yun - Biotechnology Advances, 2021 - Elsevier
Improvement in intrinsic enzymatic features is in many instances a prerequisite for the
scalable applicability of many industrially important biocatalysts. To this end, various …

Horseradish peroxidase (HRP) as a tool in green chemistry

GR Lopes, DCGA Pinto, AMS Silva - Rsc Advances, 2014 - pubs.rsc.org
Horseradish peroxidase (HRP) is a well-known and commercially available enzyme that has
been used over the last years in a wide range of applications from which one can …

Protein modifications: From chemoselective probes to novel biocatalysts

TB Pessatti, H Terenzi, JB Bertoldo - Catalysts, 2021 - mdpi.com
Chemical reactions can be performed to covalently modify specific residues in proteins.
When applied to native enzymes, these chemical modifications can greatly expand the …

Colorimetric paper bioassay by horseradish peroxidase for the detection of catechol and resorcinol in aqueous samples

A Dabhade, S Jayaraman… - Preparative biochemistry …, 2020 - Taylor & Francis
Phenolic compounds such as catechol and resorcinol are toxic and persistent pollutants in
the aqueous environment. Detection procedures such as chromatographic and …

Directly quantifiable biotinylation using a water-soluble isatoic anhydride platform

AB Fessler, AJ Fowler, CA Ogle - Bioconjugate Chemistry, 2021 - ACS Publications
Isatoic anhydride (IA) has been shown to be a useful platform for quantifiable
bioconjugation. The elaboration of a water-soluble isatoic anhydride-based platform with …

An accomplished procedure of horseradish peroxidase immobilization for removal of acid yellow 11 in aqueous solutions

M Altikatoglu Yapaoz, A Attar - Water Science and Technology, 2020 - iwaponline.com
Horseradish peroxidase (HRP) characteristics were improved by two techniques, Na-
alginate entrapment and glutaraldehyde crosslinking prior to alginate entrapment, in order to …

Investigating the effect of gallium curcumin and gallium diacetylcurcumin complexes on the structure, function and oxidative stability of the peroxidase enzyme and …

P Jahangoshaei, L Hassani, F Mohammadi… - JBIC Journal of …, 2015 - Springer
Curcumin has a wide spectrum of biological and pharmacological activities including anti-
inflammatory, antioxidant, antiproliferative, antimicrobial and anticancer activities …

Chemically modified magnetic immobilized phospholipase A1 and its application for soybean oil degumming

W Weining, H Tang, Y Chen, J Liu, D Yu… - Journal of Food Science …, 2022 - Springer
In this paper, the free Phospholipase A 1 (PLA 1) was immobilized on a magnetic carrier.
The average particle diameter of the magnetic carrier was 97±1.3 nm, and the average …

The biological effects of vanadyl curcumin and vanadyl diacetylcurcumin complexes: the effect on structure, function and oxidative stability of the peroxidase enzyme …

A Hamidi, L Hassani, F Mohammadi… - Journal of enzyme …, 2016 - Taylor & Francis
Curcumin has multiple pharmacological effects, but it has poor stability. Complexation of
curcumin with metals improves its stability. Here, the effects of vanadyl curcumin and …