Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation

G Zhao, D Lu, M Li, Y Wang - Frontiers in Microbiology, 2023 - frontiersin.org
Mycoplasmas are successful pathogens that cause debilitating diseases in humans and
various animal hosts. Despite the exceptionally streamlined genomes, mycoplasmas have …

Comprehensive quantitative modeling of translation efficiency in a genome‐reduced bacterium

M Weber, A Sogues, E Yus, R Burgos… - Molecular Systems …, 2023 - embopress.org
Translation efficiency has been mainly studied by ribosome profiling, which only provides an
incomplete picture of translation kinetics. Here, we integrated the absolute quantifications of …

Engineering a genome‐reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo

V Garrido, C Piñero‐Lambea… - Molecular Systems …, 2021 - embopress.org
Bacteria present a promising delivery system for treating human diseases. Here, we
engineered the genome‐reduced human lung pathogen Mycoplasma pneumoniae as a live …

SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome

C Pinero-Lambea, E Garcia-Ramallo… - Nucleic Acids …, 2022 - academic.oup.com
The development of advanced genetic tools is boosting microbial engineering which can
potentially tackle wide-ranging challenges currently faced by our society. Here we present …

Mycoplasma pneumoniae Genome Editing Based on Oligo Recombineering and Cas9-Mediated Counterselection

C Piñero-Lambea, E Garcia-Ramallo… - ACS synthetic …, 2020 - ACS Publications
Mycoplasma species share a set of features, such as lack of a cell wall, streamlined
genomes, simplified metabolism, and the use of a deviant genetic code, that make them …

A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications

A Broto, E Gaspari, S Miravet-Verde… - Nature …, 2022 - nature.com
Mycoplasmas have exceptionally streamlined genomes and are strongly adapted to their
many hosts, which provide them with essential nutrients. Owing to their relative genomic …

Improved transformation efficiency in Mycoplasma hominis enables disruption of the MIB–MIP system targeting human immunoglobulins

J Guiraud, C Le Roy, F Rideau… - Microbiology …, 2023 - Am Soc Microbiol
The pathogenicity of Mycoplasma hominis is poorly understood, mainly due to the absence
of efficient genetic tools. A polyethylene glycol-mediated transformation protocol was …

ProTInSeq: transposon insertion tracking by ultra-deep DNA sequencing to identify translated large and small ORFs

S Miravet-Verde, R Mazzolini, C Segura-Morales… - nature …, 2024 - nature.com
Identifying open reading frames (ORFs) being translated is not a trivial task. ProTInSeq is a
technique designed to characterize proteomes by sequencing transposon insertions …

Genome Engineering in Mycoplasma gallisepticum Using Exogenous Recombination Systems

T Ipoutcha, G Gourgues, C Lartigue… - ACS Synthetic …, 2022 - ACS Publications
Mycoplasma gallisepticum (Mgal) is a common pathogen of poultry worldwide that has
recently spread to North American house finches after a single host shift in 1994. The …

Imaging minimal bacteria at the nanoscale: A reliable and versatile process to perform single-molecule localization microscopy in mycoplasmas

F Rideau, A Villa, P Belzanne, E Verdier… - Microbiology …, 2022 - Am Soc Microbiol
Mycoplasmas are the smallest free-living organisms. These bacteria are important models
for both fundamental and synthetic biology, owing to their highly reduced genomes. They …