Since the early 1990s, single-molecule detection in solution at room temperature has
enabled direct observation of single biomolecules at work in real time and under …

Real‐time feedback‐driven single‐particle tracking (RT‐FD‐SPT) is a class of techniques in
the field of single‐particle tracking that uses feedback control to keep a particle of interest in …

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool to quantify molecular
compositions and study molecular states in complex cellular environment as the lifetime …

Targeted delivery and labeling of single living cells in heterogeneous cell populations are of
great importance to understand the molecular biology and physiological functions of …

Recent experiments have shown that the diffusion of reagent molecules is inconsistent with
what the Stokes–Einstein equation predicts during a chemical reaction. Here, we used …

Biomacromolecules exist and function in a crowded and spatially confined intracellular
milieu. Single-cell analysis has been an essential tool for deciphering the molecular …

Dynamic DNA nanodevices represent powerful tools for the interrogation and manipulation
of biological systems. Yet, implementation remains challenging due to nuclease degradation …

Here, we present a three-dimensional two-color dual-particle tracking (3D-2C-DPT)
technique that can simultaneously localize two spectrally distinct targets in three dimensions …

Two-photon excited fluorescence (TPEF) is a powerful technique that enables the
examination of intrinsic retinal fluorophores involved in cellular metabolism and the visual …

Real-time feedback-driven single-particle tracking is a technique that uses feedback control
to enable single-molecule spectroscopy of freely diffusing particles in native or near-native …