Sophisticated molecular genetic, biochemical and biophysical studies have been used to
probe the molecular mechanism of actomyosin-based motility. Recent solution …

New crystal structures of the kinesin motors differ from previously described motor-ADP
atomic models, showing striking changes both in the switch I region near the nucleotide …

Cytoplasmic dynein is a minus-end-directed microtubule motor whose mechanism of
movement remains poorly understood. Here, we use optical tweezers to examine the force …

Fluorine is the thirteenth most abundant element in the earth's crust, but fluoride
concentrations in surface water are low and fluorinated metabolites are extremely rare,. The …

Myosins are molecular motor proteins that harness the chemical energy stored in ATP to
produce directed force along actin filaments. Complex communication pathways link the …

Background: α-Actinin is a ubiquitously expressed protein found in numerous actin
structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C …

Human families with single amino acid mutations in nonmuscle myosin heavy chain (NMHC)
II-A (MYH9) and II-C (MYH14) have been described as have mice generated with a point …

Dynamins form a family of multidomain GTPases involved in endocytosis, vesicle trafficking
and maintenance of mitochondrial morphology. In contrast to the classical switch GTPases …

The fluorescence emission intensity from a conserved tryptophan residue (W501) located in
the relay loop (F466 to L516) of the Dicytostelium discoideum myosin II motor domain is …

We combined protein engineering and single molecule measurements to directly record the
step size of a series of myosin constructs with shortened and elongated artificial neck …