Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand
breaks (DSBs) is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and …

The detyrosination-tyrosination cycle involves the removal and religation of the C-terminal
tyrosine of α-tubulin and is implicated in cognitive, cardiac, and mitotic defects. The …

Tubulin is subjected to a number of posttranslational modifications to generate
heterogeneous microtubules. The modifications include removal and ligation of the C …

Chimeric antigen receptors (CARs) can effectively redirect cytotoxic T cells toward highly
expressed surface antigens on tumor cells. The low expression of several tumor-associated …

DNA double-strand break (DSB) repair is mediated by multiple pathways. It is thought that
the local chromatin context affects the pathway choice, but the underlying principles are …

Highlights•Different molecular cloning strategies exist for recombinant protein
expression.•Various methods for generation of mutation and deletion constructs are …

The deubiquitinating enzyme BAP1 is an important tumor suppressor that has drawn
attention in the clinic since its loss leads to a variety of cancers. BAP1 is activated by ASXL1 …

Ubiquitination is a central process affecting all facets of cellular signalling and function. A
critical step in ubiquitination is the transfer of ubiquitin from an E2 ubiquitin-conjugating …

In mammalian interphase nuclei, more than one thousand large genomic regions are
positioned at the nuclear lamina (NL). These lamina‐associated domains (LAD s) are …

Ubiquitin-specific proteases (USPs) are papain-like isopeptidases with variable inter-and
intramolecular regulatory domains. To understand the effect of these domains on USP …