Single-cell RNA sequencing to explore immune cell heterogeneity

E Papalexi, R Satija - Nature Reviews Immunology, 2018 - nature.com
Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive
analysis of the immune system. In this Review, we briefly describe the available scRNA-seq …

From haematopoietic stem cells to complex differentiation landscapes

E Laurenti, B Göttgens - Nature, 2018 - nature.com
The development of mature blood cells from haematopoietic stem cells has long served as a
model for stem-cell research, with the haematopoietic differentiation tree being widely used …

[HTML][HTML] Chromatin potential identified by shared single-cell profiling of RNA and chromatin

S Ma, B Zhang, LM LaFave, AS Earl, Z Chiang, Y Hu… - Cell, 2020 - cell.com
Cell differentiation and function are regulated across multiple layers of gene regulation,
including modulation of gene expression by changes in chromatin accessibility. However …

Single-cell analysis reveals transcriptomic remodellings in distinct cell types that contribute to human prostate cancer progression

S Chen, G Zhu, Y Yang, F Wang, YT Xiao, N Zhang… - Nature cell …, 2021 - nature.com
Prostate cancer shows remarkable clinical heterogeneity, which manifests in spatial and
clonal genomic diversity. By contrast, the transcriptomic heterogeneity of prostate tumours is …

A single‐cell RNA expression atlas of normal, preneoplastic and tumorigenic states in the human breast

B Pal, Y Chen, F Vaillant, BD Capaldo, R Joyce… - The EMBO …, 2021 - embopress.org
To examine global changes in breast heterogeneity across different states, we determined
the single‐cell transcriptomes of> 340,000 cells encompassing normal breast, preneoplastic …

[PDF][PDF] RNA-Seq signatures normalized by mRNA abundance allow absolute deconvolution of human immune cell types

G Monaco, B Lee, W Xu, S Mustafah, YY Hwang… - Cell reports, 2019 - cell.com
The molecular characterization of immune subsets is important for designing effective
strategies to understand and treat diseases. We characterized 29 immune cell types within …

[PDF][PDF] Transcriptional basis of mouse and human dendritic cell heterogeneity

CC Brown, H Gudjonson, Y Pritykin, D Deep… - Cell, 2019 - cell.com
Dendritic cells (DCs) play a critical role in orchestrating adaptive immune responses due to
their unique ability to initiate T cell responses and direct their differentiation into effector …

Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent antitumor immunity in melanoma

N Jacquelot, C Seillet, M Wang, A Pizzolla, Y Liao… - Nature …, 2021 - nature.com
Group 2 innate lymphoid cells (ILC2s) are essential to maintain tissue homeostasis. In
cancer, ILC2s can harbor both pro-tumorigenic and anti-tumorigenic functions, but we know …

[PDF][PDF] Single-cell RNA-seq reveals AML hierarchies relevant to disease progression and immunity

P van Galen, V Hovestadt, MH Wadsworth II… - Cell, 2019 - cell.com
Acute myeloid leukemia (AML) is a heterogeneous disease that resides within a complex
microenvironment, complicating efforts to understand how different cell types contribute to …

[PDF][PDF] Single-cell map of diverse immune phenotypes in the breast tumor microenvironment

E Azizi, AJ Carr, G Plitas, AE Cornish, C Konopacki… - Cell, 2018 - cell.com
Knowledge of immune cell phenotypes in the tumor microenvironment is essential for
understanding mechanisms of cancer progression and immunotherapy response. We …