Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …

The epigenome involves a complex set of cellular processes governing genomic activity.
Dissecting this complexity necessitates the development of tools capable of specifically …

The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Ischaemic heart disease is a leading cause of death worldwide. Injury to the heart is
followed by loss of the damaged cardiomyocytes, which are replaced with fibrotic scar …

Epigenome editing has rapidly evolved in recent years, with diverse applications that
include elucidating gene regulation mechanisms, annotating coding and noncoding …

Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression
of multiple genes remains challenging. We generated a transgenic mouse using an …

Determining causal relationships between distinct chromatin features and gene expression,
and ultimately cell behavior, remains a major challenge. Recent developments in targetable …

Multiplexed modulation of endogenous genes is crucial for sophisticated gene therapy and
cell engineering. CRISPR–Cas12a systems enable versatile multiple-genomic-loci targeting …

Due to the ability to regulate target metabolic pathways globally and dynamically, metabolic
regulation systems composed of transcription factors have been widely used in metabolic …

CRISPR-Cas9 technologies have dramatically increased the ease of targeting DNA
sequences in the genomes of living systems. The fusion of chromatin-modifying domains to …