Enzymatic approaches to challenges in chemical synthesis are increasingly popular and
very attractive to industry given their green nature and high efficiency compared to traditional …

Colicins are proteins produced by and toxic for some strains of Escherichia coli. They are
produced by strains of E. coli carrying a colicinogenic plasmid that bears the genetic …

Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of
yeast nuclear DNA linked to pMB 9, a derivative of the ColEl plasmid from E. coli. Two …

In vitro recombination techniques. were used to construct a new cloning vehicle, pBR322.
This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and …

This book has been written to meet the needs of students for biotechnology courses at
various levels of undergraduate and graduate studies. This book covers all the important …

We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of
Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) …

The Harvey murine sarcoma virus (Ha-MuSV) transforming gene, v-ras H, encodes a 21,000
molecular weight protein (p21) that is closely related to the p21 proteins encoded by the …

Virulence of the plant pathogen Erwinia carotovora subsp. carotovora is dependent on the
production and secretion of a complex arsenal of plant cell wall‐degrading enzymes …

I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an
Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a …

The determination of the total 5,224 base-pair DNA sequence of the virus SV40 has enabled
us to locate precisely the known genes on the genome. At least 15.2% of the genome is …