Identity, structure, and function of the mitochondrial permeability transition pore: controversies, consensus, recent advances, and future directions
The mitochondrial permeability transition (mPT) describes a Ca2+-dependent and
cyclophilin D (CypD)-facilitated increase of inner mitochondrial membrane permeability that …
cyclophilin D (CypD)-facilitated increase of inner mitochondrial membrane permeability that …
G protein-coupled receptors: structure-and function-based drug discovery
D Yang, Q Zhou, V Labroska, S Qin… - Signal transduction and …, 2021 - nature.com
As one of the most successful therapeutic target families, G protein-coupled receptors
(GPCRs) have experienced a transformation from random ligand screening to knowledge …
(GPCRs) have experienced a transformation from random ligand screening to knowledge …
Structure of the TRPA1 ion channel suggests regulatory mechanisms
The TRPA1 ion channel (also known as the wasabi receptor) is a detector of noxious
chemical agents encountered in our environment or produced endogenously during tissue …
chemical agents encountered in our environment or produced endogenously during tissue …
Molecular signatures of G-protein-coupled receptors
G-protein-coupled receptors (GPCRs) are physiologically important membrane proteins that
sense signalling molecules such as hormones and neurotransmitters, and are the targets of …
sense signalling molecules such as hormones and neurotransmitters, and are the targets of …
The styrene–maleic acid copolymer: a versatile tool in membrane research
JM Dörr, S Scheidelaar, MC Koorengevel… - European Biophysics …, 2016 - Springer
A new and promising tool in membrane research is the detergent-free solubilization of
membrane proteins by styrene–maleic acid copolymers (SMAs). These amphipathic …
membrane proteins by styrene–maleic acid copolymers (SMAs). These amphipathic …
A general protocol for the generation of Nanobodies for structural biology
E Pardon, T Laeremans, S Triest, SGF Rasmussen… - Nature protocols, 2014 - nature.com
There is growing interest in using antibodies as auxiliary tools to crystallize proteins. Here
we describe a general protocol for the generation of Nanobodies to be used as …
we describe a general protocol for the generation of Nanobodies to be used as …
Screening and large-scale expression of membrane proteins in mammalian cells for structural studies
Structural, biochemical and biophysical studies of eukaryotic membrane proteins are often
hampered by difficulties in overexpression of the candidate molecule. Baculovirus …
hampered by difficulties in overexpression of the candidate molecule. Baculovirus …
Crystal structure of the β2 adrenergic receptor–Gs protein complex
G protein-coupled receptors (GPCRs) are responsible for the majority of cellular responses
to hormones and neurotransmitters as well as the senses of sight, olfaction and taste. The …
to hormones and neurotransmitters as well as the senses of sight, olfaction and taste. The …
Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore
The molecular identity of the mitochondrial megachannel (MMC)/permeability transition pore
(PTP), a key effector of cell death, remains controversial. By combining highly purified, fully …
(PTP), a key effector of cell death, remains controversial. By combining highly purified, fully …
Structure and dynamics of the M3 muscarinic acetylcholine receptor
Acetylcholine, the first neurotransmitter to be identified, exerts many of its physiological
actions via activation of a family of G-protein-coupled receptors (GPCRs) known as …
actions via activation of a family of G-protein-coupled receptors (GPCRs) known as …