Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions

A Sharma, SM Shah, M Tiwari, M Roshan, MK Singh… - Cytotechnology, 2020 - Springer
In the present study, we used a serum-free culture media to propagate goat putative
spermatogonial stem cells (SSCs) and evaluated the effect of crucial growth factors on …

Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock

B Binsila, S Selvaraju, R Ranjithkumaran… - Journal of Assisted …, 2021 - Springer
Abstract Purpose Spermatogonial stem cells (SSCs) are the source for the mature male
gamete. SSC technology in humans is mainly focusing on preserving fertility in cancer …

Establishment of effective and safe recipient preparation for germ-cell transplantation with intra-testicular busulfan treatment in pre-pubertal Barbari goats

SP Singh, SD Kharche, M Pathak, YK Soni… - Theriogenology, 2022 - Elsevier
The busulfan, an alkylating agent, suppresses endogenous spermatogenesis in recipient
testes. However, considering a wide variation in the effects of busulfan among animal …

Successful transplantation of transfected enriched buffalo (Bubalus bubalis) spermatogonial stem cells to homologous recipients

A Sharma, A Kumaresan, P Mehta, N Nala, MK Singh… - Theriogenology, 2020 - Elsevier
Genetic modification of spermatogonial stem cells (SSCs) is an alternative method to
pronuclear microinjection and somatic cell nuclear transfer for transgenesis in large animals …

Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT

P Mehta, R Kaushik, KP Singh, A Sharma, MK Singh… - Theriogenology, 2019 - Elsevier
Somatic cell nuclear transfer (SCNT), using transgenic donor cells, is a highly efficient
method for producing transgenic embryos. We compared the developmental competence …

Culture bovine prospermatogonia with 2i medium

H Cai, Y Jiang, S Zhang, NN Cai, WQ Zhu, R Yang… - Andrologia, 2021 - Wiley Online Library
Germplasm cryopreservation and expansion of gonocytes/prospermatogonia or
spermatogonial stem cells (SSCs) are important; however, it's difficult in cattle. Since …

[PDF][PDF] 干细胞技术在肉牛育种中的研究进展及应用前景

陈瑞, 陈欢, 段安琴, 李孟琪, 冯超, 尚江华 - 中国畜禽种业, 2024 - xq.aiijournal.com
摘要院牛肉作为主要优质肉类蛋白来源之一袁其市场需求正随着人口增长而增加遥通过传统
育种方法培育良种牛无法满足这种日益增长的需要袁利用现代生物技术进行肉牛育种势在必行 …

Spermatogonial stem cells: their use in fertility restoration

A Sharma, A Kumaresan, P Vats… - Frontier Technologies in …, 2022 - Springer
Spermatogonial stem cells (SSCs) are the only adult stem cells in males capable of
transmitting their genetic information to the next generation. Over the past two decades …

Spermatogonial stem cells and testis-tissue cryopreservation as a tool for conservation of buffalo germplasm

L Devi, S Goel - Biotechnological Applications in Buffalo Research, 2022 - Springer
Buffalo is an economically valuable animal of the south-east Asian counties, and they have
made a significant contribution to livestock production. In recent days, due to the rapid …

Propagation of porcine spermatogonial stem cells in serum‐free culture conditions using knockout serum replacement

A Das, D Bhuyan, T Lalmalsawma… - … in Domestic Animals, 2023 - Wiley Online Library
In vitro culture and expansion of spermatogonial stem cells (SSCs) is an essential
prerequisite to enhancing livestock productivity through SSC transplantation. Most of the …