In this post-genomic era, we need to define gene function on a genome-wide scale for
model organisms and humans. The fundamental unit of biological processes is the cell …

Target-based high-throughput screening (HTS) has recently been critiqued for its relatively
poor yield compared to phenotypic screening approaches. One type of phenotypic …

Antibody-based proteomics provides a powerful approach for the functional study of the
human proteome involving the systematic generation of protein-specific affinity reagents. We …

Imaging techniques such as immunofluorescence (IF) and the expression of fluorescent
protein (FP) fusions are widely used to investigate the subcellular distribution of proteins …

Dramatic advances in methods for protein tagging and the development of fully automated
microscopes enable collection of unprecedented volumes of image data on the subcellular …

Here, we describe a robust protocol for the reverse transfection of cells on small interfering
(siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse …

Developments in subcellular fractionation strategies have provided the means to profile and
analyze the protein composition of organelles and cellular structures by proteomics. Here …

Fluorescence microscopy is a powerful method to study protein function in its natural habitat,
the living cell. With the availability of the green fluorescent protein and its spectral variants …

Breast cancer is a hormone-dependent cancer and usually treated with endocrine therapy
using aromatase inhibitors or anti-estrogens such as tamoxifen. A majority of breast cancer …

The endoplasmic reticulum (ER) regulates organelle dynamics through the formation of
membrane contact sites (MCS). Here we describe that VMP1, a multispanning ER-resident …