Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022 - pubs.rsc.org
Enzyme immobilization has been developing since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Enzyme immobilization and co-immobilization: main framework, advances and some applications

J Bié, B Sepodes, PCB Fernandes, MHL Ribeiro - Processes, 2022 - mdpi.com
Enzymes are outstanding (bio) catalysts, not solely on account of their ability to increase
reaction rates by up to several orders of magnitude but also for the high degree of substrate …

[HTML][HTML] Enzyme immobilization strategies for the design of robust and efficient biocatalysts

JM Guisan, G Fernandez-Lorente… - Current Opinion in …, 2022 - Elsevier
Different strategies for the preparation of efficient and robust immobilized biocatalysts are
here reviewed. Different physico-chemical approaches are discussed. i.-The stabilization of …

Transcriptomic analysis of Raphidocelis subcapitata exposed to erythromycin: The role of DNA replication in hormesis and growth inhibition

J Guo, Z Ma, J Peng, J Mo, Q Li, J Guo… - Journal of Hazardous …, 2021 - Elsevier
The occurrence of hormesis in the algal growth inhibition test is a major challenge in the
dose-response characterization, whereas the molecular mechanism remains unraveled. The …

From enzyme stability to enzymatic bioelectrode stabilization processes

C Beaufils, HM Man, A de Poulpiquet, I Mazurenko… - Catalysts, 2021 - mdpi.com
Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing,
electricity production, or biosynthesis of fine products. Despite advances in the knowledge of …

Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl …

NS Mohammadi, MS Khiabani, B Ghanbarzadeh… - Journal of …, 2020 - Elsevier
In this study, the factors affecting lipase adsorption onto SiO 2 nanoparticles including SiO 2
nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL) …

[HTML][HTML] Intensification of oxygen-dependent biotransformations catalyzed by immobilized enzymes

A Lorente-Arevalo, M Ladero, JM Bolivar - Current Opinion in Green and …, 2021 - Elsevier
Highlights•Intensification of oxygen-dependent biotransformations relies on a complex
interplay of catalyst/reaction/reactor.•Enzyme immobilization is a useful technology to …

Use of potential immobilized enzymes for the modification of liquid foods in the food industry

E Garcia-Quinto, R Aranda-Cañada, P García-García… - Processes, 2023 - mdpi.com
Enzymes are complex proteins that carry out biochemical reactions. Apart from being
necessary for life, they are used in numerous industrial processes, especially in the textile …

Immobilization of the Peroxygenase from Agrocybe aegerita. The Effect of the Immobilization pH on the Features of an Ionically Exchanged Dimeric Peroxygenase

D Carballares, R Morellon-Sterling, X Xu, F Hollmann… - Catalysts, 2021 - mdpi.com
This paper outlines the immobilization of the recombinant dimeric unspecific peroxygenase
from Agrocybe aegerita (r Aae UPO). The enzyme was quite stable (remaining unaltered its …