Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies

H Yamaguchi, M Miyazaki - Biomolecules, 2014 - mdpi.com
Biologically active proteins are useful for studying the biological functions of genes and for
the development of therapeutic drugs and biomaterials in a biotechnology industry …

Micro total analysis systems. Latest advancements and trends

PS Dittrich, K Tachikawa, A Manz - Analytical chemistry, 2006 - ACS Publications
The area of micro total analysis systems (μTAS), also called “lab on a chip”, miniaturized or
microfluidic analysis systems, is a rapidly developing field. During the last several years, we …

Microfluidic mixing and the formation of nanoscale lipid vesicles

A Jahn, SM Stavis, JS Hong, WN Vreeland… - ACS …, 2010 - ACS Publications
We investigate the formation of unilamellar lipid vesicles (liposomes) with diameters of tens
of nanometers by controlled microfluidic mixing and nanoparticle determination …

Microfluidic hydrodynamic focusing for synthesis of nanomaterials

M Lu, A Ozcelik, CL Grigsby, Y Zhao, F Guo, KW Leong… - Nano Today, 2016 - Elsevier
Microfluidics expands the synthetic space such as heat transfer, mass transport, and reagent
consumption to conditions not easily achievable in conventional batch processes …

Single-molecule fluorescence studies of protein folding and conformational dynamics

X Michalet, S Weiss, M Jäger - Chemical reviews, 2006 - ACS Publications
Single-molecule measurements provide unique information on heterogeneous populations
of molecules: They give access to the complete distribution of observables (rather than only …

Microfluidic and lab-on-a-chip preparation routes for organic nanoparticles and vesicular systems for nanomedicine applications

L Capretto, D Carugo, S Mazzitelli, C Nastruzzi… - Advanced drug delivery …, 2013 - Elsevier
In recent years, advancements in the fields of microfluidic and lab-on-a-chip technologies
have provided unique opportunities for the implementation of nanomaterial production …

Electrokinetic mixing in microfluidic systems

CC Chang, RJ Yang - Microfluidics and Nanofluidics, 2007 - Springer
The applications of electrokinetics in the development of microfluidic devices have been
widely attractive in the past decade. Electrokinetic devices generally require no external …

Single-molecule FRET for probing nanoscale biomolecular dynamics

D Nettels, N Galvanetto, MT Ivanović, M Nüesch… - Nature Reviews …, 2024 - nature.com
Single-molecule spectroscopy is a powerful method for studying the physics of molecular
systems, particularly biomolecules, such as proteins and nucleic acids. By avoiding …

Droplet microfluidics for food and nutrition applications

K Schroen, C Berton-Carabin, D Renard, M Marquis… - Micromachines, 2021 - mdpi.com
Droplet microfluidics revolutionizes the way experiments and analyses are conducted in
many fields of science, based on decades of basic research. Applied sciences are also …

Protein folding transition path times from single molecule FRET

HS Chung, WA Eaton - Current opinion in structural biology, 2018 - Elsevier
Highlights•Single molecule FRET experiments determine average transition path
times.•Observed times are extremely close to those found in all-atom MD simulations.•Times …