Great interests have recently been aroused in the independent associative domain of
glycoside hydrolases that utilize insoluble polysaccharides-carbohydrate-binding module …

Literature from the past two decades has outlined the existence of a trade-off between
protein stability and function. This trade-off creates a unique challenge for protein engineers …

The development of precise and modulated methods for customized manipulation of DNA is
an important objective for the study and engineering of biological processes and is essential …

Background Saccharification of lignocellulosic material by xylanases and other glycoside
hydrolases is generally conducted at high concentrations of the final reaction products …

All biosensing platforms rest on two pillars: specific biochemical recognition of a particular
analyte and transduction of that recognition into a readily detectable signal. Most existing …

Used as model for sandwich fusion, a mesophilic Aspergillus niger GH11 xylanase (Xyn)
was fused into C2-Xyn-C2 with a thermophilic Thermotaga maritima GH10 xylanase …

Catalase (CAT) plays a crucial role in plant responses to environmental stresses and
maintaining redox homeostasis. However, its putative heat lability might compromise its …

Background Glucoamylase is an important industrial enzyme for the saccharification of
starch during sugar production, but the production cost of glucoamylase is a major limiting …

High xylanase activity and stability toward alkaline pH is strongly desired for pulping and
bleaching processes. We previously enhanced thermal stability of Bacillus circulans …

High thermostability of an enzyme is critical for its industrial application. While many
engineering approaches such as mutagenesis have enhanced enzyme thermostability, they …