In this comprehensive review, I focus on the twenty E. coli aminoacyl‐tRNA synthetases and
their ability to charge non‐canonical amino acids (ncAAs) onto tRNAs. The promiscuity of …

The bacteria-derived tyrosyl-tRNA synthetase (TyrRS)/tRNA pair was first used for unnatural
amino acid (Uaa) mutagenesis in eukaryotic cells over 15 years ago. It provides an ideal …

Suppressor transfer RNAs (sup-tRNAs) are receiving renewed attention for their promising
therapeutic properties in treating genetic diseases caused by nonsense mutations …

Amino acids have a multi-billion-dollar market with rising demand, prompting the
development of high-performance microbial factories. However, a general screening …

Synthesis of proteins with noncanonical amino acids (ncAAs) enables the creation of protein-
based biomaterials with diverse new chemical properties that may be attractive for material …

The relative quantitative importance of the factors that determine the fidelity of translation is
largely unknown, which makes predicting the extent to which the degeneracy of the genetic …

Reprogramming of the genetic code system is limited by the difficulty in creating new tRNA
structures. Here, I developed translationally active tRNA variants tagged with a small hairpin …

The genetic code of organisms can be expanded by introducing orthogonal translation
systems (OTSs). One of the most commonly applied OTSs in mammalian cells is the …

Biochemistry of Synthetic Biology - Recent Developments Biochemistry of Synthetic Biology -
Recent Developments Biochim Biophys Acta Gen Subj. 2017 Nov;1861(11 Pt B):2945-2947 …