[HTML][HTML] Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global …

KM Ruppert, RJ Kline, MS Rahman - Global Ecology and Conservation, 2019 - Elsevier
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity
wherein samples are taken from the environment via water, sediment or air from which DNA …

[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems

J Pawlowski, M Kelly-Quinn, F Altermatt… - Science of the Total …, 2018 - Elsevier
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …

Meta‐analysis shows that environmental DNA outperforms traditional surveys, but warrants better reporting standards

J Fediajevaite, V Priestley, R Arnold… - Ecology and …, 2021 - Wiley Online Library
Decades of environmental DNA (eDNA) method application, spanning a wide variety of taxa
and habitats, has advanced our understanding of eDNA and underlined its value as a tool …

The expanding role of DNA barcodes: Indispensable tools for ecology, evolution, and conservation

MR Gostel, WJ Kress - Diversity, 2022 - mdpi.com
DNA barcoding has transformed the fields of ecology, evolution, and conservation by
providing a rapid and effective tool for species identification. The growth of DNA barcodes as …

DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers

OS Wangensteen, C Palacín, M Guardiola, X Turon - PeerJ, 2018 - peerj.com
Biodiversity assessment of marine hard-bottom communities is hindered by the high
diversity and size-ranges of the organisms present. We developed a DNA metabarcoding …

Validation of COI metabarcoding primers for terrestrial arthropods

V Elbrecht, TWA Braukmann, NV Ivanova, SWJ Prosser… - PeerJ, 2019 - peerj.com
Metabarcoding can rapidly determine the species composition of bulk samples and thus
aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that …

A comparison of droplet digital polymerase chain reaction (PCR), quantitative PCR and metabarcoding for species‐specific detection in environmental DNA

SA Wood, X Pochon, O Laroche… - Molecular Ecology …, 2019 - Wiley Online Library
Targeted species‐specific and community‐wide molecular diagnostics tools are being used
with increasing frequency to detect invasive or rare species. Few studies have compared the …

Benchmarking DNA metabarcoding for biodiversity-based monitoring and assessment

E Aylagas, Á Borja, X Irigoien… - Frontiers in Marine …, 2016 - frontiersin.org
Characterization of biodiversity has been extensively used to confidently monitor and assess
environmental status. Yet, visual morphology, traditionally and widely used for species …