Synergistic advances in optical physics, probe design, molecular biology, labeling
techniques and computational analysis have propelled fluorescence imaging into new …

Sleep quality declines with age; however, the underlying mechanisms remain elusive. We
found that hyperexcitable hypocretin/orexin (Hcrt/OX) neurons drive sleep fragmentation …

To achieve its precise neural connectivity, the developing mammalian nervous system
undergoes extensive activity-dependent synapse remodelling. Recently, microglial cells …

The circadian clock and homeostatic processes are fundamental mechanisms that regulate
sleep. Surprisingly, despite decades of research, we still do not know why we sleep …

Synapses of the mammalian CNS are diverse in size, structure, molecular composition, and
function. Synapses in their myriad variations are fundamental to neural circuit development …

Chronic treatment with fluoxetine (FLX) is required for its antidepressant effects, but the role
of serotonin (5-HT) axonal plasticity in FLX action is unknown. To address this, we examined …

The quality of super resolution images obtained by stochastic single-molecule microscopy
critically depends on image analysis algorithms. We find that the choice of background …

Selective serotonin (5-HT) reuptake inhibitors are only 30% effective for remission in
subjects with major depression, and the best treatments for SSRI-resistant patients remain …

Thalamus is a potent driver of cortical activity even though cortical synapses onto excitatory
layer 4 neurons outnumber thalamic synapses 10 to 1. Previous in vitro studies have …

Serotonin neurons arise from the brainstem raphe nuclei and send their projections
throughout the brain to release 5-HT which acts as a modulator of several neuronal …