Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses

F Pasin, W Menzel, JA Daròs - Plant Biotechnology Journal, 2019 - Wiley Online Library
Recent metagenomic studies have provided an unprecedented wealth of data, which are
revolutionizing our understanding of virus diversity. A redrawn landscape highlights viruses …

Development of a reverse genetic system for studying rose rosette virus in whole plants

J Verchot, V Herath, CD Urrutia… - Molecular Plant …, 2020 - Am Phytopath Society
Rose rosette virus (RRV) is a negative-sense RNA virus with a seven-segmented genome
that is enclosed by a double membrane. We constructed an unconventional minireplicon …

Rapid Construction of Complex Plant RNA Virus Infectious cDNA Clones for Agroinfection Using a Yeast-E. coli-Agrobacterium Shuttle Vector

K Sun, D Zhao, Y Liu, C Huang, W Zhang, Z Li - Viruses, 2017 - mdpi.com
The availability of infectious full-length clone is indispensable for reverse genetics studies of
virus biology, pathology and construction of viral vectors. However, for RNA viruses with …

Rapid construction of stable infectious full-length cDNA clone of papaya leaf distortion mosaic virus using in-fusion cloning

D Tuo, W Shen, P Yan, X Li, P Zhou - Viruses, 2015 - mdpi.com
Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic
papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of …

A simple, rapid and efficient way to obtain infectious clones of potyviruses

C Desbiez, C Chandeysson, H Lecoq… - Journal of virological …, 2012 - Elsevier
The availability of an infectious cDNA clone is a prerequisite for genetic studies on RNA
viruses. However, despite important improvement in molecular biology techniques during …

Fluorescently Tagged Potato virus Y: A Versatile Tool for Functional Analysis of Plant-Virus Interactions

M Rupar, F Faurez, M Tribodet… - Molecular Plant …, 2015 - Am Phytopath Society
Potato virus Y (PVY) is an economically important plant virus that infects Solanaceous crops
such as tobacco and potato. To date, studies into the localization and movement of PVY in …

Complete genome sequencing and construction of full-length infectious cDNA clone of papaya ringspot virus-HYD isolate and its efficient in planta expression

P Gupta, PLC Parupudi, L Supriya… - Frontiers in …, 2023 - frontiersin.org
Papaya ringspot virus (PRSV) is a devastating Potyvirus that causes papaya ringspot
disease in Carica papaya plantations globally. In this study, the complete genome sequence …

Strategies for the construction of cassava brown streak disease viral infectious clones

CRA Duff-Farrier, DR Mbanzibwa, S Nanyiti… - Molecular …, 2019 - Springer
Cassava brown streak disease (CBSD) has major impacts on yield and quality of the
tuberous roots of cassava in Eastern and Central Arica. At least two Potyviridae species …

[HTML][HTML] Complete genome sequencing and infectious cdna clone construction of soybean mosaic virus isolated from Shanxi

D Wang, L Cui, L Zhang, Z Ma, Y Niu - The Plant Pathology Journal, 2021 - ncbi.nlm.nih.gov
Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and
quality of soybean. The natural host range for SMV is very narrow, and generally limited to …

Molecular characterization of potyviruses infecting potato and vegetables in Iraq

NA Al-Kuwaiti, MN Maruthi, SE Seal - Journal of Plant Pathology, 2016 - JSTOR
The genetic diversity of potyviruses infecting potato and vegetables in Iraq was determined
by reverse transcription polymerase chain reaction (RT-PCR) targeting the 3 terminal part of …