Single-cell DNA methylation analysis in cancer

H O'Neill, H Lee, I Gupta, EJ Rodger, A Chatterjee - Cancers, 2022 - mdpi.com
Simple Summary Cancer is a distinctly difficult disease to treat on account of the diverse cell
populations/subpopulations that comprise a tumour. Such cells harbour varying genetic and …

Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes

H Jia, S Tan, Y Cai, Y Guo, J Shen, Y Zhang… - Nature …, 2024 - nature.com
Long-read sequencing, exemplified by PacBio, revolutionizes genomics, overcoming
challenges like repetitive sequences. However, the high DNA requirement (> 1 µg) is …

Benchmarking DNA isolation methods for marine metagenomics

A Demkina, D Slonova, V Mamontov, O Konovalova… - Scientific reports, 2023 - nature.com
Metagenomics is a powerful tool to study marine microbial communities. However, obtaining
high-quality environmental DNA suitable for downstream sequencing applications is a …

A discovery down under: decoding the draft genome sequence of Pantoea stewartii from Australia's Critically Endangered western ground parrot/kyloring (Pezoporus …

RT White, W Taylor, N Klukowski… - Microbial …, 2023 - microbiologyresearch.org
Pantoea stewartii, a plant pathogen, is primarily transmitted through contaminated seeds
and insect vectors, with the corn flea beetle (Chaetocnema pulicaria) being the primary …

NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A rapid, PCR-free method for SARS-CoV-2 whole-genome sequencing in clinical samples

MA Terrazos Miani, L Borcard, S Gempeler… - Pathogens, 2024 - mdpi.com
Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage
characterization in diagnostic laboratories worldwide. The rapid, near-full-length sequencing …

Reducing DNA extraction costs through factorial design for the DNAdvance Kit

CJ Neal, ZD Zbinden, ME Douglas, MR Douglas - BMC Research Notes, 2024 - Springer
Objective Extracting DNA is essential in wildlife genetic studies, and numerous methods are
available. However, the process is costly and time-consuming for non-model organisms …

[HTML][HTML] Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing

AC Simpson, S Tighe, S Wong, P Leo… - Journal of …, 2023 - ncbi.nlm.nih.gov
The rapid assessment of microbiomes from ultra-low biomass environments such as
cleanrooms or hospital operating rooms has a number of applications for human health and …

Maximizing the potential of genomic and transcriptomic studies by nanopore sequencing

D Meyer, W Göttsch, J Spannenberg, P Bohn, B Stieber… - bioRxiv, 2023 - biorxiv.org
Nucleic acid sequencing is the process of identifying the sequence of DNA or RNA, with
DNA used for genomes and RNA for transcriptomes. Deciphering this information has the …

A high-quality de novo genome assembly based on nanopore sequencing of a wild-caught coconut rhinoceros beetle (Oryctes rhinoceros)

I Filipović, G Rašić, J Hereward, M Gharuka, GJ Devine… - BMC genomics, 2022 - Springer
Background An optimal starting point for relating genome function to organismal biology is a
high-quality nuclear genome assembly, and long-read sequencing is revolutionizing the …

Benchmarking DNA Isolation Methods for Marine Metagenomics Studies

A Demkina, D Slonova, V Mamontov, O Konovalova… - bioRxiv, 2023 - biorxiv.org
Metagenomics is a powerful tool to study marine microbial communities. However, obtaining
high-quality environmental DNA suitable for downstream sequencing applications is a …