Microglia states and nomenclature: A field at its crossroads

RC Paolicelli, A Sierra, B Stevens, ME Tremblay… - Neuron, 2022 - cell.com
Microglial research has advanced considerably in recent decades yet has been constrained
by a rolling series of dichotomies such as" resting versus activated" and" M1 versus M2." …

Extracellular vesicle analysis

A Hendrix, L Lippens, C Pinheiro, C Théry… - Nature Reviews …, 2023 - nature.com
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

Identification of best housekeeping genes for the normalization of RT-qPCR in human cell lines

L da Conceição Braga, BÔP Gonçalves, PL Coelho… - Acta …, 2022 - Elsevier
The identification of the best reference gene is a critical step to evaluate the relative change
in mRNA expression of a target gene by RT-qPCR. In this work, we evaluated nineteen …

[HTML][HTML] Gastrointestinal symptoms and fecal shedding of SARS-CoV-2 RNA suggest prolonged gastrointestinal infection

A Natarajan, S Zlitni, EF Brooks, SE Vance, A Dahlen… - Med, 2022 - cell.com
Background COVID-19 manifests with respiratory, systemic, and gastrointestinal (GI)
symptoms. 1, SARS-CoV-2 RNA is detected in respiratory and fecal samples, and recent …

Soybean photosynthesis and crop yield are improved by accelerating recovery from photoprotection

AP De Souza, SJ Burgess, L Doran, J Hansen… - Science, 2022 - science.org
Crop leaves in full sunlight dissipate damaging excess absorbed light energy as heat. This
protective dissipation continues after the leaf transitions to shade, reducing crop …

First detection of SARS-CoV-2 RNA in wastewater in North America: a study in Louisiana, USA

SP Sherchan, S Shahin, LM Ward, S Tandukar… - Science of The Total …, 2020 - Elsevier
We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated …

Evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as coronavirus disease 2019 (COVID-19) pandemic: A global health emergency

T Acter, N Uddin, J Das, A Akhter, TR Choudhury… - Science of the Total …, 2020 - Elsevier
According to data compiled by researchers at Johns Hopkins University in Baltimore,
Maryland, more than two and half million cases of coronavirus disease 2019 (COVID-19) …

Parameters for successful PCR primer design

SA Bustin, R Mueller, T Nolan - Quantitative Real-Time PCR: Methods and …, 2020 - Springer
Primers are critical components of any PCR assay, as they are the main determinants of its
specificity, sensitivity, and robustness. Despite the publication of numerous guidelines, the …

The biogenesis, biology and characterization of circular RNAs

LS Kristensen, MS Andersen, LVW Stagsted… - Nature reviews …, 2019 - nature.com
Circular RNAs (circRNAs) are covalently closed, endogenous biomolecules in eukaryotes
with tissue-specific and cell-specific expression patterns, whose biogenesis is regulated by …