Perturbations of native membrane protein structure in alkyl phosphocholine detergents: a critical assessment of NMR and biophysical studies
Membrane proteins perform a host of vital cellular functions. Deciphering the molecular
mechanisms whereby they fulfill these functions requires detailed biophysical and structural …
mechanisms whereby they fulfill these functions requires detailed biophysical and structural …
Allosteric regulation of SERCA by phosphorylation-mediated conformational shift of phospholamban
M Gustavsson, R Verardi, DG Mullen… - Proceedings of the …, 2013 - National Acad Sciences
The membrane protein complex between the sarcoplasmic reticulum Ca2+-ATPase
(SERCA) and phospholamban (PLN) controls Ca2+ transport in cardiomyocytes, thereby …
(SERCA) and phospholamban (PLN) controls Ca2+ transport in cardiomyocytes, thereby …
Probing membrane protein ground and conformationally excited states using dipolar-and J-coupling mediated MAS solid state NMR experiments
T Gopinath, G Veglia - Methods, 2018 - Elsevier
The intrinsic conformational plasticity of membrane proteins directly influences the
magnitude of the orientational-dependent NMR interactions such as dipolar couplings (DC) …
magnitude of the orientational-dependent NMR interactions such as dipolar couplings (DC) …
Structural dynamics and topology of phosphorylated phospholamban homopentamer reveal its role in the regulation of calcium transport
Phospholamban (PLN) inhibits the sarco (endo) plasmic reticulum Ca 2+-ATPase (SERCA),
thereby regulating cardiac diastole. In membranes, PLN assembles into homopentamers …
thereby regulating cardiac diastole. In membranes, PLN assembles into homopentamers …
[HTML][HTML] Effects of naturally occurring arginine 14 deletion on phospholamban conformational dynamics and membrane interactions
VV Vostrikov, KJ Soller, KN Ha, T Gopinath… - Biochimica et Biophysica …, 2015 - Elsevier
Phospholamban (PLN) is a single-pass membrane protein that regulates the sarco (endo)
plasmic reticulum Ca 2+-ATPase (SERCA). Phosphorylation of PLN at Ser16 reverses its …
plasmic reticulum Ca 2+-ATPase (SERCA). Phosphorylation of PLN at Ser16 reverses its …
Proton-detected polarization optimized experiments (POE) using ultrafast magic angle spinning solid-state NMR: Multi-acquisition of membrane protein spectra
T Gopinath, G Veglia - Journal of Magnetic Resonance, 2020 - Elsevier
Proton-detected solid-state NMR (ssNMR) spectroscopy has dramatically improved the
sensitivity and resolution of fast magic angle spinning (MAS) methods. While relatively …
sensitivity and resolution of fast magic angle spinning (MAS) methods. While relatively …
Structures of the excited states of phospholamban and shifts in their populations upon phosphorylation
Phospholamban is an integral membrane protein that controls the calcium balance in
cardiac muscle cells. As the function and regulation of this protein require the active …
cardiac muscle cells. As the function and regulation of this protein require the active …
[HTML][HTML] Effects of the Arg9Cys and Arg25Cys mutations on phospholamban's conformational equilibrium in membrane bilayers
SED Nelson, KN Ha, T Gopinath, MH Exline… - … et Biophysica Acta (BBA …, 2018 - Elsevier
Abstract Approximately, 70% of the Ca 2+ ion transport into the sarcoplasmic reticulum is
catalyzed by the sarcoplasmic reticulum Ca 2+-ATPase (SERCA), whose activity is …
catalyzed by the sarcoplasmic reticulum Ca 2+-ATPase (SERCA), whose activity is …
Probing the Conformationally Excited States of Membrane Proteins via 1H-Detected MAS Solid-State NMR Spectroscopy
T Gopinath, SED Nelson, KJ Soller… - The Journal of Physical …, 2017 - ACS Publications
Proteins exist in ensembles of conformational states that interconvert on various motional
time scales. High-energy states of proteins, often referred to as conformationally excited …
time scales. High-energy states of proteins, often referred to as conformationally excited …
Structural dynamics and conformational equilibria of SERCA regulatory proteins in membranes by solid-state NMR restrained simulations
Solid-state NMR spectroscopy is emerging as a powerful approach to determine structure,
topology, and conformational dynamics of membrane proteins at the atomic level …
topology, and conformational dynamics of membrane proteins at the atomic level …