In vitro techniques are very useful for conserving plant biodiversity, including (a) genetic
resources of recalcitrant seed and vegetatively propagated species,(b) rare and endangered …

Advances in plant biotechnology provide new options for collection, multiplication and short-
to long-term conservation of plant biodiversity, using in vitro culture techniques. Significant …

In vitro plant cells, tissues and organ cultures are not fully autotrophic establishing a need for
carbohydrates in culture media to maintain the osmotic potential, as well as to serve as …

This paper discusses the importance of the successive steps of the vitrification technique
and reviews the current development and use of vitrification and of the two derived …

In vitro techniques have found increasing use in the conservation of threatened plants in
recent years and this trend is likely to continue as more species face risk of extinction. The …

Cryopreservation is the storage of viable cells, tissues, organs and organisms at ultra-low
temperatures, usually in liquid nitrogen to a minimum temperature of− 196° C. The term …

Over the past decades, plant cryopreservation technologies have been evolving rapidly,
opening the door to the possibility of long-term storage of valuable genetic resources of …

Many plants cannot vitrify themselves because they lack glassy state-inducing substances
and/or have high water content. Therefore, cryoprotectants are used to induce vitrification. A …

Abstract The US Department of Agriculture-Agricultural Research Service (USDA-ARS),
National Plant Germplasm System (NPGS) plant collections are a critical source of genetic …

An alarming proportion of Australia's unique plant biodiversity is under siege from a variety
of environmental threats. Options for in situ conservation are becoming increasingly …