Antimicrobial resistance (AMR) is a global crisis for human public health which threatens the
effective prevention and control of ever-increasing infectious diseases. The advent of …

Monoderm bacteria possess a cell envelope made of a cytoplasmic membrane and a cell
wall, whereas diderm bacteria have and extra lipid layer, the outer membrane, covering the …

Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …

Lytic enzymes encoded by bacteriophages have been intensively explored as alternative
agents for combating bacterial pathogens in different contexts. The antibacterial character of …

Bacterial pathogens are leading causes of infections with high mortality worldwide having a
great impact on healthcare systems and the food industry. Gold standard methods for …

Bacteriophage‐encoded endolysins can recognize and bind specific bacteria, and act to
cleave the glycosidic and/or amide bonds in the peptidoglycan (PG) bacterial cell wall …

Bacteriophage endolysins have the potential to be a long-term antibacterial replacement for
antibiotics. The exogenous application of endolysins on some bacteria results in rapid cell …

Mycobacteriophages are viruses that specifically infect mycobacteria, which ultimately
culminate in host cell death. Dedicated enzymes targeting the complex mycobacterial cell …

Using bacteriophage-derived endolysins as an alternative strategy for fighting drug-resistant
bacteria has recently been garnering renewed interest. However, their application is still …

Highlights•Codon optimization can dramatically boost protein expression.•Replacement of
rare codons can affect protein folding.•Synthetic gene design would benefit from novel …