Fluorescence lifetime imaging microscopy

B Torrado, B Pannunzio, L Malacrida… - Nature Reviews …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

Label‐Free Assessment of Key Biological Autofluorophores: Material Characteristics and Opportunities for Clinical Applications

JM Campbell, M Gosnell, A Agha, S Handley… - Advanced …, 2024 - Wiley Online Library
Autofluorophores are endogenous fluorescent compounds that naturally occur in the intra
and extracellular spaces of all tissues and organs. Most have vital biological functions–like …

Light-field tomographic fluorescence lifetime imaging microscopy

Y Ma, J Park, L Huang, C Sen, S Burri… - Proceedings of the …, 2024 - pnas.org
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that
enables the visualization of biological samples at the molecular level by measuring the …

Physical, biomechanical, and optical characterization of collagen and elastin blend hydrogels

N Vazquez-Portalatin, A Alfonso-Garcia, JC Liu… - Annals of biomedical …, 2020 - Springer
Collagen and elastin proteins are major components of the extracellular matrix of many
organs. The presence of collagen and elastin networks, and their associated properties, in …

Direct frequency domain fluorescence lifetime imaging using field programmable gate arrays for real time processing

MJ Serafino, BE Applegate, JA Jo - Review of Scientific Instruments, 2020 - pubs.aip.org
Frequency domain (FD) fluorescence lifetime imaging (FLIM) involves the excitation of the
sample of interest with a modulated light source and digitization of the fluorescence …

Luminescence lifetime imaging using a cellphone camera with an electronic rolling shutter

B Xiong, Q Fang - Optics Letters, 2019 - opg.optica.org
Luminescence lifetime imaging systems are typically complex and expensive instruments
targeting bench-top applications. We present a low-cost approach for video frame rate …

Naphthopyran-based photoswitching for simpler chemical sensing and imaging using phase-sensitive mode

Y Cheng, Y Wang, J Zhai, X Xie - Cell Reports Physical Science, 2024 - cell.com
Intensity-based fluorescence imaging suffers from spectral overlap and optical background
interference. As an alternative, fluorescence lifetime measurements on the nanosecond …

A Frequency-domain optofluidic dissolved oxygen sensor with total internal reflection design for in situ monitoring

B Xiong, E Mahoney, JF Lo… - IEEE Journal of Selected …, 2020 - ieeexplore.ieee.org
Continuous measurement of dissolved oxygen (DO) variation is important in water
monitoring and biomedical applications, which require low-cost and low-maintenance …

Direct frequency domain fluorescence lifetime imaging using simultaneous ultraviolet and visible excitation

MJ Serafino, JA Jo - Biomedical Optics Express, 2023 - opg.optica.org
Due to the complexity, limited practicality, and cost of conventional fluorescence lifetime
imaging/microscopy (FLIM) instrumentation, FLIM adoption has been mostly limited to …

Imaging of scleral collagen deformation using combined confocal raman microspectroscopy and polarized light microscopy techniques

N Chakraborty, M Wang, J Solocinski, W Kim… - PLoS …, 2016 - journals.plos.org
This work presents an optospectroscopic characterization technique for soft tissue
microstructure using site-matched confocal Raman microspectroscopy and polarized light …