Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Over the past decade, remarkable advances have been witnessed in the development of
small-molecule probes. These molecular tools have been widely applied for interrogating …

Cells in complex organisms undergo frequent functional changes, but few methods allow
comprehensive longitudinal profiling of living cells. Here we introduce scission-accelerated …

Bioorthogonal chemical reactions are a thriving area of chemical research in recent years as
an unprecedented technique to dissect native biological processes through chemistry …

Uncovering the mechanisms of virus infection and assembly is crucial for preventing the
spread of viruses and treating viral disease. The technique of single-virus tracking (SVT) …

Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Since the establishment of site-specific mutagenesis of single amino acids to interrogate
protein function in the 1970s, biochemists have sought to tailor protein structure in the native …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …