Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Acting as a bridge between the cytoskeleton of the cell and the extra cellular matrix (ECM),
the cell-ECM adhesions with integrins at their core, play a major role in cell signalling to …

Fluorogenic probes efficiently reduce non-specific background signals, which often results in
highly improved signal-to-noise ratios. Although this implies improved resolution …

Super‐resolution microscopy (SRM) greatly benefits from the ability to install small
photostable fluorescent labels into proteins. Genetic code expansion (GCE) technology …

Highlights•The potential impact of multi-color single-molecule FRET imaging.•Key
requirement: ultra-stable fluorophores spanning the visible spectrum.•Intramolecularly …

Super-resolution imaging by single-molecule localization (localization microscopy) provides
the ability to unravel the structural organization of cells and the composition of biomolecular …

Super-resolution fluorescence imaging by single-molecule photoactivation or
photoswitching and position determination (localization microscopy) has the potential to …

The clustering of membrane receptors such as EGFR is critical for various biological
processes, for example cell signaling and tumorigenesis. However, the mechanism involved …

We designed and synthesized a new pH fluorescent probe, RCE, based on structural
changes of rhodamine dye at different pH values. The probe exhibits high selectivity, high …