Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

miRNAs can be found in serum and other body fluids and serve as biomarkers for disease.
More importantly, secreted miRNAs, especially those in extracellular vesicles (EVs) such as …

Primers are critical components of any PCR assay, as they are the main determinants of its
specificity, sensitivity, and robustness. Despite the publication of numerous guidelines, the …

Wastewater surveillance for pathogens using reverse transcription-polymerase chain
reaction (RT-PCR) is an effective and resource-efficient tool for gathering community-level …

MicroRNAs (miRNAs) are a class of small noncoding RNAs, which function in
posttranscriptional regulation of gene expression. They are powerful regulators of various …

Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods
have revolutionized environmental microbiology, yielding quantitative organism-specific …

Abstract Background Environmental DNA (eDNA) analysis is increasingly being used to
detect the presence and relative abundance of rare species, especially invasive or imperiled …

MicroRNAs (miRNAs) are short endogenously expressed RNAs that have the potential to
regulate the expression of any RNA. This potential has led to the publication of several …

Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

Bioindication has become an indispensable part of water quality monitoring in most
countries of the world, with the presence and abundance of bioindicator taxa, mostly …