Integration of Agrobacterium T-DNA into the Plant Genome

SB Gelvin - Annual review of genetics, 2017 - annualreviews.org
Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir)
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …

Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

SB Gelvin - Microbiology and molecular biology reviews, 2003 - Am Soc Microbiol
Agrobacterium tumefaciens and related Agrobacterium species have been known as plant
pathogens since the beginning of the 20th century. However, only in the past two decades …

The silence of genes in transgenic plants

M Stam, JNM Mol, JM Kooter - Annals of botany, 1997 - Elsevier
In genetically modified plants, the introduced transgenes are sometimes not expressed.
They can be silenced. Transgenes can also cause the silencing of endogenous plant genes …

Transgene inactivation: plants fight back!

J Finnegan, D McElroy - Bio/technology, 1994 - nature.com
Desirable new phenotypes created by the introduction of foreign DNA into plants are
frequently unstable following propagation, leading to a loss of the newly acquired traits. This …

[HTML][HTML] Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA

SM Angell, DC Baulcombe - The EMBO Journal, 1997 - embopress.org
Tobacco plants were transformed with constructs in which the transgene was a cDNA of
replicating potato virus X (PVX) RNA. The constructs, referred to here as amplicons, were …

Approaches to minimize variation of transgene expression in plants

KMJ Butaye, BPA Cammue, SL Delauré… - Molecular …, 2005 - Springer
Genetic transformation of plants has become a widely used technology that serves multiple
purposes in plant biology research. However, considerable variation of transgene …

Use of matrix attachment regions (MARs) to minimize transgene silencing

GC Allen, S Spiker, WF Thompson - Plant Gene Silencing, 2000 - Springer
Matrix attachment regions (MARs) are operationally defined as DNA elements that bind
specifically to the nuclear matrix in vitro. It is possible, although unproven, that they also …

Homologous pairing and chromosome dynamics in meiosis and mitosis

BD McKee - Biochimica et Biophysica Acta (BBA)-Gene Structure …, 2004 - Elsevier
Pairing of homologous chromosomes is an essential feature of meiosis, acting to promote
high levels of recombination and to ensure segregation of homologs. However, homologous …

High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco.

GC Allen, G Hall Jr, S Michalowski, W Newman… - The Plant …, 1996 - academic.oup.com
We have previously shown that yeast scaffold attachment regions (SARs) flanking a chimeric
beta-glucuronidase (GUS) reporter gene increased per-copy expression levels by 24-fold in …

Stable high‐level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions

KMJ Butaye, IJWM Goderis, PFJ Wouters… - The plant …, 2004 - Wiley Online Library
Basic and applied research involving transgenic plants often requires consistent high‐level
expression of transgenes. However, high inter‐transformant variability of transgene …