Cellular mechanics during division of a genomically minimal cell

JF Pelletier, JI Glass, EA Strychalski - Trends in Cell Biology, 2022 - cell.com
Genomically minimal cells, such as JCVI-syn3. 0 and JCVI-syn3A, offer an empowering
framework to study relationships between genotype and phenotype. With a polygenic basis …

Evolution of longitudinal division in multicellular bacteria of the Neisseriaceae family

S Nyongesa, PM Weber, È Bernet, F Pulido… - Nature …, 2022 - nature.com
Rod-shaped bacteria typically elongate and divide by transverse fission. However, several
bacterial species can form rod-shaped cells that divide longitudinally. Here, we study the …

Miniscule Mollicutes: Current hurdles to bacteriophage identification

J Catchpowle, J Maynard, BJ Chang… - Sustainable …, 2024 - academic.oup.com
Mollicutes are a diverse class of bacteria with a variety of unique characteristics that have
allowed them to adapt to a range of hosts and often evade routine cultivation techniques …

Toward the complete functional characterization of a minimal bacterial proteome

DM Bianchi, JF Pelletier, CA Hutchison III… - The Journal of …, 2022 - ACS Publications
Recently, we presented a whole-cell kinetic model of the genetically minimal bacterium JCVI-
syn3A that described the coupled metabolic and genetic information processes and …

Septal wall synthesis is sufficient to change ameba-like cells into uniform oval-shaped cells in Escherichia coli L-forms

M Hayashi, C Takaoka, K Higashi, K Kurokawa… - Communications …, 2024 - nature.com
A cell wall is required to control cell shape and size to maintain growth and division.
However, some bacterial species maintain their morphology and size without a cell wall …

Imaging minimal bacteria at the nanoscale: A reliable and versatile process to perform single-molecule localization microscopy in mycoplasmas

F Rideau, A Villa, P Belzanne, E Verdier… - Microbiology …, 2022 - Am Soc Microbiol
Mycoplasmas are the smallest free-living organisms. These bacteria are important models
for both fundamental and synthetic biology, owing to their highly reduced genomes. They …

MraZ transcriptionally controls the critical level of FtsL required for focusing Z-rings and Kickstarting Septation in Bacillus subtilis

ML White, A Hough-Neidig, SJ Khan… - Journal of …, 2022 - Am Soc Microbiol
The bacterial division and cell wall (dcw) cluster is a highly conserved region of the genome
which encodes several essential cell division factors, including the central divisome protein …

Genome editing of veterinary relevant mycoplasmas using a CRISPR-Cas base editor system

T Ipoutcha, F Rideau, G Gourgues, Y Arfi… - Applied and …, 2022 - Am Soc Microbiol
Mycoplasmas are minimal bacteria that infect humans, wildlife, and most economically
relevant livestock species. Mycoplasma infections cause a large range of chronic …

Taxonomic Re-Classification and Expansion of the Phylum Chloroflexota Based on over 5000 Genomes and Metagenome-Assembled Genomes

S Wiegand, M Sobol, LK Schnepp-Pesch, G Yan… - Microorganisms, 2023 - mdpi.com
The phylum Chloroflexota (formerly Chloroflexi) encompasses metabolically diverse
bacteria that often have high prevalence in terrestrial and aquatic habitats, some even with …

The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock

G Merker Breyer, A Malvessi Cattani… - Molecular Biology …, 2022 - Springer
Background The comprehension of genome organization and gene modulation is essential
for understanding pathogens' infection mechanisms. Mycoplasma hyopneumoniae 7448 …