Purification and biochemical characterization of phytocystatin from Brassica alba

A Ahmed, A Shamsi, B Bano - Journal of Molecular Recognition, 2016 - Wiley Online Library
Phytocystatins belong to the family of cysteine proteinases inhibitors. They are ubiquitously
found in plants and carry out various significant physiological functions. These plant derived …

Structural and functional studies on a variant of cystatin purified from brain of Capra hircus

PSS Khaki, A Feroz, F Amin, MT Rehman… - Journal of …, 2017 - Taylor & Francis
Cystatins, known for their ubiquitous presence in mammalian system are thiol protease
inhibitors serving important physiological functions. Here, we present a variant of cystatin …

Purification and characterization of high molecular mass and low molecular mass cystatin from goat brain

S Sumbul, B Bano - Neurochemical research, 2006 - Springer
Cystatin are thiol proteinase inhibitors ubiquitously present in mammalian body and serve
various important physiological functions. In the present study two cystatins were isolated …

Cystatin like thiol proteinase inhibitor from pancreas of Capra hircus: purification and detailed biochemical characterization

M Priyadarshini, B Bano - Amino Acids, 2010 - Springer
A thiol proteinase inhibitor from Capra hircus (goat) pancreas (PTPI) isolated by ammonium
sulphate precipitation (20–80%) and gel filtration chromatography on Sephacryl S-100HR …

Purification and characterizaion of buffalo brain cystatin

F Amin, A Ali Khan, S Jahan Rizvi… - Protein and Peptide …, 2011 - ingentaconnect.com
Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve
various important physiological functions. Aims: To purify and characterize Thiol protease …

Conformational transitions induced by in vitro macromolecular crowding lead to the amyloidogenesis of buffalo heart cystatin

A Sohail, SA Bhat, AA Siddiqui… - Journal of Molecular …, 2015 - Wiley Online Library
The biological cells and extracellular matrix exhibit a highly crowded environment, called as
macromolecular crowding. Crowding significantly influences protein structure and may lead …

Physico-chemical and in-silico analysis of a phytocystatin purified from Brassica juncea cultivar RoAgro 5444

S Khan, S Ahmad, MI Siddiqi… - Biochemistry and Cell …, 2016 - cdnsciencepub.com
This study describes the isolation and purification of a phytocystatin from seeds of Brassica
juncea (Indian mustard; cultivar RoAgro 5444), which is an important oilseed crop both …

Biochemical, immunological and kinetic characterization and partial sequence analysis of a thiol proteinase inhibitor from Bubalus bubalis kidney: An attempt targeting …

A Shamsi, A Ahmed, B Bano - International journal of biological …, 2017 - Elsevier
In the present study a thiol proteinase inhibitor was isolated from buffalo kidney making use
of ammonium sulphate precipitation and gel filtration chromatography on Sephacryl S …

Surface Plasmon Resonance Imaging biosensor for cystatin determination based on the application of bromelain, ficin and chymopapain

E Gorodkiewicz, J Breczko… - Folia Histochemica et …, 2012 - journals.viamedica.pl
Abstract A Surface Plasmon Resonance Imaging (SPRI) sensor based on bromelain or
chymopapain or ficin has been developed for specific cystatin determination. Cystatin was …

Three low molecular weight cysteine proteinase inhibitors of human seminal fluid: purification and enzyme kinetic properties

VK Yadav, N Chhikara, K Gill, S Dey, S Singh, S Yadav - Biochimie, 2013 - Elsevier
The cystatins form a superfamily of structurally related proteins with highly conserved
structural folds. They are all potent, reversible, competitive inhibitors of cysteine proteinases …