Polymerase chain reaction in microfluidic devices

CD Ahrberg, A Manz, BG Chung - Lab on a Chip, 2016 - pubs.rsc.org
The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular
biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules …

Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications

L Cao, X Cui, J Hu, Z Li, JR Choi, Q Yang, M Lin… - Biosensors and …, 2017 - Elsevier
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a
significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and …

Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells

Y Zhu, PD Piehowski, R Zhao, J Chen, Y Shen… - Nature …, 2018 - nature.com
Nanoscale or single-cell technologies are critical for biomedical applications. However,
current mass spectrometry (MS)-based proteomic approaches require samples comprising a …

Droplet microarrays: from surface patterning to high‐throughput applications

W Feng, E Ueda, PA Levkin - Advanced materials, 2018 - Wiley Online Library
High‐throughput screening of live cells and chemical reactions in isolated droplets is an
important and growing method in areas ranging from studies of gene functions and the …

Nanoliter-scale oil-air-droplet chip-based single cell proteomic analysis

ZY Li, M Huang, XK Wang, Y Zhu, JS Li… - Analytical …, 2018 - ACS Publications
Single cell proteomic analysis provides crucial information on cellular heterogeneity in
biological systems. Herein, we describe a nanoliter-scale oil-air-droplet (OAD) chip for …

Miniaturizing chemistry and biology using droplets in open systems

Y Zeng, JW Khor, TL van Neel, W Tu… - Nature Reviews …, 2023 - nature.com
Open droplet microfluidic systems manipulate droplets on the picolitre-to-microlitre scale in
an open environment. They combine the compartmentalization and control offered by …

Recent advances in the use of microfluidic technologies for single cell analysis

TW Murphy, Q Zhang, LB Naler, S Ma, C Lu - Analyst, 2018 - pubs.rsc.org
The inherent heterogeneity in cell populations has become of great interest and importance
as analytical techniques have improved over the past decades. With the advent of …

Bioinspired superwettable micropatterns for biosensing

T Xu, LP Xu, X Zhang, S Wang - Chemical Society Reviews, 2019 - pubs.rsc.org
The bioinspired micropatterns exhibit outstanding capacity in controlling and patterning
microdroplets, which have offered new functionalities and possibilities towards a wide …

Bioprinting a cardiac valve

S Jana, A Lerman - Biotechnology advances, 2015 - Elsevier
Heart valve tissue engineering could be a possible solution for the limitations of mechanical
and biological prostheses, which are commonly used for heart valve replacement. In tissue …

Bio-inspired wettability patterns for biomedical applications

J Chi, X Zhang, Y Wang, C Shao, L Shang… - Materials Horizons, 2021 - pubs.rsc.org
Benefiting from the remarkable wettability heterogeneity, bio-inspired wettability patterns
present a progressive and versatile platform for manipulating and patterning liquids, which …