Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …

Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for
decades, but the recent breaking of the diffraction resolution barrier by sequentially …

In this Perspective we discuss recent trends in the development and applications of
fluorescent proteins. We start by providing a historical and structural perspective of their …

Highlights•Photoswitchable fluorescent proteins cycle between on and off states in response
to light.•Structural studies reveal diversity in the details of photoswitching …

Photochromic materials are a family of compounds which undergo photo-reversible
transformations between two different isomers with distinct physical and chemical properties …

Phototransformable fluorescent proteins (FPs) have received considerable attention in
recent years, because they enable many new exciting modalities in fluorescence …

The fluorescent protein (FP) color palette has greatly contributed to the visualization of
molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than …

Super-resolution fluorescence microscopy has become an invaluable, powerful approach to
study biomolecular dynamics and interactions via selective labeling and observation of …

Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores
with controllable emission properties. Chief among these fluorophores are the …