Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins

PJ Mitchell, R Tjian - Science, 1989 - science.org
The cloning of genes encoding mammalian DNA binding transcription factors for RNA
polymerase II has provided the opportunity to analyze the structure and function of these …

Enhancers, enhancers–from their discovery to today's universe of transcription enhancers

W Schaffner - Biological Chemistry, 2015 - degruyter.com
Abstract Transcriptional enhancers are short (200–1500 base pairs) DNA segments that are
able to dramatically boost transcription from the promoter of a target gene. Originally …

Rolling replication of short DNA circles.

A Fire, SQ Xu - Proceedings of the National Academy of …, 1995 - National Acad Sciences
Natural genes and proteins often contain tandemly repeated sequence motifs that
dramatically increase physiological specificity and activity. Given the selective value of such …

The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants

PN Benfey, NH Chua - Science, 1990 - science.org
Appropriate regulation of transcription in higher plants requires specific cis elements in the
regulatory regions of genes and their corresponding trans-acting proteins. Analysis of the …

The c-fos protein interacts with c-JunAP-1 to stimulate transcription of AP-1 responsive genes

R Chiu, WJ Boyle, J Meek, T Smeal, T Hunter, M Karin - Cell, 1988 - cell.com
Cell lines stably transfected with metal inducible, MT-fos chimeric genes were used to study
the ability of the c-fos gene product, Fos, to act as a transcriptional trans-activator. In …

Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements

W Lee, P Mitchell, R Tjian - Cell, 1987 - cell.com
The enhancer-binding protein AP-1 has been purified to> 95% homogeneity from HeLa cells
by sequencespecific DNA affinity chromatography and identified as a 47 kd polypeptide …

The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue‐specific expression patterns

PN Benfey, L Ren, NH Chua - The EMBO journal, 1989 - embopress.org
We have analyzed expression conferred by two domains from the cauliflower mosaic virus
(CaMV) 35S promoter and found different patterns in seeds, seedlings and seven week old …

Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1

JH Xiao, I Davidson, H Matthes, JM Garnier… - Cell, 1991 - cell.com
We describe the cDNA encoding the SV40 transcriptional enhancer factor 1 (TEF-1) and
show that its translation initiates exclusively at an AUU codon in vivo. Cloned TEF-1, which …

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

RX Fang, F Nagy, S Sivasubramaniam… - The Plant Cell, 1989 - academic.oup.com
The 35S promoter is a major promoter of the cauliflower mosaic virus that infects crucifers.
This promoter is still active when excised from cauliflower mosaic virus and integrated into …

Tissue‐specific expression from CaMV 35S enhancer subdomains in early stages of plant development.

PN Benfey, L Ren, NH Chua - The EMBO journal, 1990 - embopress.org
The cauliflower mosaic virus (CaMV) 35S enhancer is able to confer strong constitutive
expression in plants. We have previously defined two domains within this enhancer that can …