Double-stranded RNA-mediated interference (RNAi) is a simple and rapid method of
silencing gene expression in a range of organisms. The silencing of a gene is a …

Toxoplasma gondii is an obligate intracellular protozoan parasite which is a significant
human and veterinary pathogen. Other members of the phylum Apicomplexa are also …

Protozoan parasites that profoundly affect mankind represent an exceptionally diverse group
of organisms, including Plasmodium, Toxoplasma, Entamoeba, Giardia, trypanosomes, and …

Many intracellular pathogens are separated from the cytosol of their host cells by a vacuole
membrane. This membrane serves as a critical interface between the pathogen and the host …

In Toxoplasma gondii, lactate dehydrogenase is encoded by two independent and
developmentally regulated genes LDH1 and LDH2. These genes and their products have …

Publisher Summary The first genetic manipulations applied to Toxoplasma were performed
by using chemical mutagenesis. The reverse genetics approach, which introduces foreign …

The complex life cycles of many protozoan parasites require the ability to respond to
environmental and developmental cues through regulated gene expression. Traditionally …

Entamoeba histolytica, a protozoan parasite with variable DNA content and complex ploidity,
has defied most efforts aimed at gene depletion using classical genetic methods. In this …

Protozoan pathogens have evolved countermeasures to avoid immune clearance and
prolong the period of infection in their vertebrate hosts. The type and degree of immune …

Intracellular protozoan parasites are a great threat to animal and human health. To
successfully disseminate through an organism these parasites must be able to enter and exit …