Endogenous ion channels expressed in human embryonic kidney (HEK-293) cells

J Zhang, H Yuan, X Yao, S Chen - Pflügers Archiv-European Journal of …, 2022 - Springer
Mammalian expression systems, particularly the human embryonic kidney (HEK-293) cells,
combined with electrophysiological studies, have greatly benefited our understanding of the …

[HTML][HTML] Endogenous channels in HEK cells and potential roles in HCN ionic current measurements

A Varghese, EM TenBroek, J Coles Jr… - Progress in biophysics and …, 2006 - Elsevier
A transformed line of human embryonic kidney epithelial cells (HEK 293) is commonly used
as an expression system for exogenous ion channel genes. Previously, it has been shown …

Cdk5-dependent phosphorylation of CaV3. 2 T-type channels: possible role in nerve ligation-induced neuropathic allodynia and the compound action potential in …

K Gomez, A Calderón-Rivera, A Sandoval… - Journal of …, 2020 - Soc Neuroscience
Voltage-gated T-type Ca2+ (CaV3) channels regulate diverse physiological events,
including neuronal excitability, and have been linked to several pathological conditions such …

Calcium signaling stimulates translation of HIF‐α during hypoxia

AS Hui, AL Bauer, JB Striet, PO Schnell… - The FASEB …, 2006 - Wiley Online Library
Hypoxia‐inducible factors (HIFs) are ubiquitous transcription factors that mediate adaptation
to hypoxia by inducing specific sets of target genes. It is well accepted that hypoxia induces …

Proximal clustering between BK and CaV1.3 channels promotes functional coupling and BK channel activation at low voltage

O Vivas, CM Moreno, LF Santana, B Hille - Elife, 2017 - elifesciences.org
CaV-channel dependent activation of BK channels is critical for feedback control of both
calcium influx and cell excitability. Here we addressed the functional and spatial interaction …

Stable gastric pentadecapeptide BPC 157 and bupivacaine

G Zivanovic-Posilovic, D Balenovic, I Barisic… - European journal of …, 2016 - Elsevier
Bupivacaine toxicity following accidental overdose still lacks therapeutic solution. However,
there are major arguments for testing BPC 157 against bupivacaine toxicity in vivo in rats, in …

Identification of a disulfide bridge essential for structure and function of the voltage-gated Ca2+ channel α2δ-1 auxiliary subunit

A Calderón-Rivera, A Andrade… - Cell calcium, 2012 - Elsevier
Voltage-gated calcium (CaV) channels are transmembrane proteins that form Ca2+-
selective pores gated by depolarization and are essential regulators of the intracellular …

The expression of endogenous voltage‐gated potassium channels in HEK293 cells is affected by culture conditions

A Ponce, A Castillo, L Hinojosa… - Physiological …, 2018 - Wiley Online Library
HEK 293 cells are widely used as a host for expression of heterologous proteins; yet, little
care has been taken to characterize their endogenous membrane components, including …

Regulation of L-type CaV1. 3 channel activity and insulin secretion by the cGMP-PKG signaling pathway

A Sandoval, P Duran, MA Gandini, A Andrade… - Cell Calcium, 2017 - Elsevier
Abstract cGMP is a second messenger widely used in the nervous system and other tissues.
One of the major effectors for cGMP is the serine/threonine protein kinase, cGMP-dependent …

CaV2.2 channel cell surface expression is regulated by the light chain 1 (LC1) of the microtubule-associated protein B (MAP1B) via UBE2L3-mediated ubiquitination …

MA Gandini, DR Henríquez, L Grimaldo… - … -European Journal of …, 2014 - Springer
Microtubule-associated protein B is a cytoskeleton protein consisting of heavy and light (LC)
chains that play important roles in the regulation of neuronal morphogenesis and function …