Genome engineering has revolutionized several scientific fields, ranging from biochemistry
and fundamental research to therapeutic uses and crop development. Diverse engineering …

The type VK CRISPR-associated transposons (CASTs) allow RNA-guided DNA integration
and have great potential as a programmable site-specific gene insertion tool. Although all …

A long time has passed since regularly interspaced DNA repeats were discovered in
prokaryotes. Today, those enigmatic repetitive elements termed clustered regularly …

The bacterium Escherichia coli has been well-justified as an effective workhorse for
industrial applications. In this study, we developed a toolkit for flexible genome engineering …

The INTEGRATE system is a gene‐editing approach that offers advantages over the widely
used CRISPR‐Cas9 system. It does not introduce double strand breaks in the target DNA …

CRISPR‐Cas class 1 includes types I, III, and IV. All class 1 systems have typical
multisubunit complexes, often with a Cas nuclease, either stand‐alone (Cas3 in type I) or …

Bacteria live under constant assault by bacteriophages and have evolved a diverse array of
defense strategies. CRISPR-Cas systems are prokaryotic adaptive immune systems that rely …

Recent research has revealed a collaboration between type IF CRISPR systems and Tn7
transposons in certain bacteria, leading to the discovery of a new gene-editing tool called …

The bacterium Escherichia coli has been well-justified as an effective workhorse for
industrial applications. In this study, we developed a toolkit for flexible genome engineering …