Next generation and other sequencing technologies in diagnostic microbiology and infectious diseases

EE Hilt, P Ferrieri - Genes, 2022 - mdpi.com
Next-generation sequencing (NGS) technologies have become increasingly available for
use in the clinical microbiology diagnostic environment. There are three main applications of …

Current uses and future perspectives of genomic technologies in clinical microbiology

I Bianconi, R Aschbacher, E Pagani - Antibiotics, 2023 - mdpi.com
Recent advancements in sequencing technology and data analytics have led to a
transformative era in pathogen detection and typing. These developments not only expedite …

Fungal whole-genome sequencing for species identification: from test development to clinical utilization

Z Salem-Bango, TK Price, JL Chan, S Chandrasekaran… - Journal of Fungi, 2023 - mdpi.com
Using next-generation sequencing (NGS), we developed and validated a whole-genome
sequencing (WGS)-based clinical test for fungal species identification on clinical isolates …

Public health implementation of pathogen genomics: the role for accreditation and application of ISO standards

SA Ballard, NL Sherry, BP Howden - Microbial Genomics, 2023 - microbiologyresearch.org
Pathogen genomics has transitioned rapidly from the research setting into a powerful tool
now routinely used in public health microbiology, for surveillance, outbreak investigations …

[HTML][HTML] The association of Lactococcus petauri with lactococcosis is older than expected

AI Vela, M del Mar Blanco, S Colussi, C Kotzamanidis… - Aquaculture, 2024 - Elsevier
Lactococcosis is a globally prevalent infectious disease that has a significant economic and
sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been …

Novel Organism Verification and Analysis (NOVA) study: identification of 35 clinical isolates representing potentially novel bacterial taxa using a pipeline based on …

V Muigg, HMB Seth-Smith, KM Adam, M Weisser… - BMC microbiology, 2024 - Springer
Background Reliable species identification of cultured isolates is essential in clinical
bacteriology. We established a new study algorithm named NOVA–N ovel O rganism V …

Performance of 16S rRNA Gene Next-Generation Sequencing and the Culture Method in the Detection of Bacteria in Clinical Specimens

A Botan, G Campisciano, V Zerbato, S Di Bella… - Diagnostics, 2024 - mdpi.com
Effective treatment of infectious diseases requires prompt and accurate bacterial
identification and tailored antimicrobial treatments. Traditional culture methods are …

Use of metagenomic next-generation sequencing in the clinical microbiology laboratory: A step forward, but not an end-all

CW Stratton, TE Schutzbank, YW Tang - The Journal of Molecular …, 2021 - jmdjournal.org
Metagenomic next-generation sequencing (mNGS) combined with bioinformatics is a
molecular-based process 1 e3 in which DNA and/or RNA are amplified and sequenced in a …

Whole-genome-sequence-based characterization of an NDM-5-producing uropathogenic Escherichia coli EC1390

TTD Thuy, HF Lu, PY Kuo, WH Lin, TP Lin, YT Lee… - BMC microbiology, 2022 - Springer
Background Urinary tract infection (UTI) is one of the most common outpatient bacterial
infections. In this study, we isolated and characterized an extensively-drug resistant (XDR) …

Clinical next-generation sequencing assay combining full-length gene amplification and shotgun sequencing for the detection of CMV drug resistance mutations

B von Bredow, JR Caldera, S Cerón, JL Chan… - Journal of Clinical …, 2023 - Elsevier
Cytomegalovirus (CMV) causes severe systemic and tissue-invasive disease in
immunocompromised patients, particularly solid organ and hematopoietic stem cell …