Synthetic biology of extremophiles: a new wave of biomanufacturing

JW Ye, YN Lin, XQ Yi, ZX Yu, X Liu, GQ Chen - Trends in Biotechnology, 2023 - cell.com
Microbial biomanufacturing, powered by the advances of synthetic biology, has attracted
growing interest for the production of diverse products. In contrast to conventional microbes …

Platforms, advances, and technical challenges in virus-like particles-based vaccines

R Gupta, K Arora, SS Roy, A Joseph… - Frontiers in …, 2023 - frontiersin.org
Viral infectious diseases threaten human health and global stability. Several vaccine
platforms, such as DNA, mRNA, recombinant viral vectors, and virus-like particle-based …

Classes and phyla of the kingdom Fungi

NN Wijayawardene, KD Hyde, KV Mikhailov, G Péter… - Fungal diversity, 2024 - Springer
Fungi are one of the most diverse groups of organisms with an estimated number of species
in the range of 2–3 million. The higher-level ranking of fungi has been discussed in the …

Precision cellular agriculture: The future role of recombinantly expressed protein as food

JH Dupuis, LKY Cheung, L Newman… - … Reviews in Food …, 2023 - Wiley Online Library
Cellular agriculture is a rapidly emerging field, within which cultured meat has attracted the
majority of media attention in recent years. An equally promising area of cellular agriculture …

Challenges and opportunities in C1-based biomanufacturing

W Qiao, S Xu, Z Liu, X Fu, H Zhao, S Shi - Bioresource Technology, 2022 - Elsevier
The intensifying impact of green-house gas (GHG) emission on environment and climate
change has attracted increasing attention, and biorefinery represents one of the most …

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

J Gao, N Gao, X Zhai, YJ Zhou - Iscience, 2021 - cell.com
Methanol biotransformation can expand biorefinery substrate spectrum other than biomass
by using methylotrophic microbes. Ogataea (Hansenula) polymorpha, a representative …

Expression of eukaryotic membrane proteins in eukaryotic and prokaryotic hosts

A Kesidis, P Depping, A Lodé, A Vaitsopoulou, RM Bill… - Methods, 2020 - Elsevier
The production of membrane proteins of high purity and in satisfactory yields is crucial for
biomedical research. Due to their involvement in various cellular processes, membrane …

A repackaged CRISPR platform increases homology-directed repair for yeast engineering

D Ploessl, Y Zhao, M Cao, S Ghosh, C Lopez… - Nature Chemical …, 2022 - nature.com
Inefficient homology-directed repair (HDR) constrains CRISPR–Cas9 genome editing in
organisms that preferentially employ nonhomologous end joining (NHEJ) to fix DNA double …

Yeast synthetic biology for designed cell factories producing secretory recombinant proteins

EJ Thak, SJ Yoo, HY Moon, HA Kang - FEMS yeast research, 2020 - academic.oup.com
Yeasts are prominent hosts for the production of recombinant proteins from industrial
enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways …

Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts

J Ruchala, AA Sibirny - FEMS Microbiology Reviews, 2021 - academic.oup.com
Pentose sugars are widespread in nature and two of them, D-xylose and L-arabinose
belong to the most abundant sugars being the second and third by abundance sugars in dry …