With increasing global food demands in the face of challenging biotic and abiotic pressures
on crop production, there is a vital need for good crop improvement strategies. Gene editing …

Efficient methods of stacking genes into plant genomes are needed to expedite transfer of
multigenic traits to crop varieties of diverse ecosystems. Over two decades of research has …

Transgene integration complexity in the recipient genome can be an important determinant
of transgene expression and field performance in transgenic crops. We provide the first …

An efficient Agrobacterium‐mediated site‐specific integration (SSI) technology using the
flipase/flipase recognition target (FLP/FRT) system in elite maize inbred lines is described …

Transgene integration mediated by heterologous site‐specific recombination (SSR) systems
into the dedicated genomic sites has been demonstrated in a few different plant species …

The last decade has seen significant strides in Agrobacterium-mediated plant transformation
technology. This has not only expanded the number of crop species that can be transformed …

Plant transformation based on random integration of foreign DNA often generates complex
integration structures. Precision in the integration process is necessary to ensure the …

Efficient methods for multigene transformation are important for developing novel crop
varieties. Methods based on random integrations of multiple genes have been successfully …

Several plant biotechnology applications are based on the expression of multiple genes
located on a single transformation vector. The principles of stable expression of foreign …

Here we review multiple uses of site-specific recombination and their applications in plant
genomic engineering. We also outline various strategies for the combined use of multiple …