Digital PCR (dPCR) is the latest technique that has become commercially accessible for
various types of research. This method uses Taq polymerase in a standard polymerase …

To the Editor: Zika virus (ZIKV), a member of the family Flaviviridae, genus Flavivirus, was
first isolated from the blood of a sentinel rhesus monkey from the Zika Forest of Uganda in …

The virus known as Japanese Encephalitis (JEV) is among the common viral persisting
Encephalitis caused by Flavivirus around the Globe, especially in Southeast Asian nations …

Dengue is usually diagnosed by isolation of the virus, serology or molecular diagnostic
methods. Several commercial kits for the diagnosis of dengue are existing, but concerns …

Background The global pork industry is continuously affected by infectious diseases that can
result in large-scale mortality, trade restrictions, and major reductions in production …

The development of technologies with rapid and sensitive detection capabilities and
increased throughput have become crucial for responding to greater number threats posed …

Bats are important reservoirs and vectors in the transmission of emerging infectious
diseases. Many highly pathogenic viruses such as SARS-CoV and rabies-related …

A one-step real-time RT-PCR assay (rRT-PCR) was developed for efficient detection of Duck
hepatitis virus type1 (DHV-1). A pair of specific primers was designed against the conserved …

Background Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia, and
the commonest cause of mosquito-borne encephalitis worldwide. Detection of JEV RNA …

Background Rapid identification and differentiation of mosquito-transmitted flaviviruses in
acute-phase sera of patients and field-caught vector mosquitoes are important for the …