The secretory production of recombinant proteins by the Gram-negative bacterium
Escherichia coli has several advantages over intracellular production as inclusion bodies. In …

Proteins that are exported from the cytoplasm to the periplasm and outer membrane of Gram-
negative bacteria, or the cell wall and growth medium of Gram-positive bacteria, are …

In bacteria, the translocase mediates the translocation of proteins into or across the cytosolic
membrane. It consists of a membrane embedded protein-conducting channel and a …

Technologies based on surface plasmon resonance (SPR) have allowed rapid, label‐free
characterization of protein‐protein and protein‐small molecule interactions. SPR has …

Most secretory proteins that are destined for the periplasm or the outer membrane are
exported through the bacterial plasma membrane by the Sec translocase. Translocase is a …

Bacterial secretory preproteins are translocated across the inner membrane post‐
translationally by the Sec YEG‐SecA translocase. Mature domain features and signal …

Secretory proteins are cotranslationally or posttranslationally translocated across lipid
membranes via a protein-conducting channel named SecY in prokaryotes and Sec61 in …

In the year 2003 there was a 17% increase in the number of publications citing work
performed using optical biosensor technology compared with the previous year. We collated …

SecA, the preprotein translocase ATPase, has a helicase DEAD motor. To catalyze protein
translocation, SecA possesses two additional flexible domains absent from other helicases …

Dual-color fluorescence-burst analysis (DCFBA) was applied to measure the quaternary
structure and high-affinity binding of the bacterial motor protein SecA to the protein …