The formation and maintenance of tissue integrity requires complex, coordinated activities
by thousands of genes and their encoded products. Until recently, transcript levels could …

Improved scale, multiplexing and resolution are establishing spatial nucleic acid and protein
profiling methods as a major pillar for cellular atlas building of complex samples, from …

Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

Gene regulation requires the dynamic coordination of hundreds of regulatory factors at
precise genomic and RNA targets. Although many regulatory factors have specific affinity for …

Identifying the relationships between chromosome structures, nuclear bodies, chromatin
states and gene expression is an overarching goal of nuclear-organization studies …

Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

In eukaryotes, the genome does not exist as a linear molecule but instead is hierarchically
packaged inside the nucleus. This complex genome organization includes multiscale …

Molecular profiling of single cells has advanced our knowledge of the molecular basis of
development. However, current approaches mostly rely on dissociating cells from tissues …

INTRODUCTION Chromatin adopts an intricate three-dimensional (3D) organization in the
nucleus that is critical for many genome processes, from gene regulation to genome …

The four-dimensional nucleome (4DN) consortium studies the architecture of the genome
and the nucleus in space and time. We summarize progress by the consortium and highlight …