Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
An efficient CRISPR–Cas12a promoter editing system for crop improvement
Promoter editing represents an innovative approach to introduce quantitative trait variation
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …
Boosting plant genome editing with a versatile CRISPR-Combo system
CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …
genome engineering in plants. However, these systems are mostly used separately, leaving …
CRISPR–Act3. 0 for highly efficient multiplexed gene activation in plants
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …
However, the simultaneous activation of multiple genes remains challenging. Here, we …
Metabolic engineering and synthetic biology strategies for producing high-value natural pigments in Microalgae
K Cao, Y Cui, F Sun, H Zhang, J Fan, B Ge, Y Cao… - Biotechnology …, 2023 - Elsevier
Microalgae are microorganisms capable of producing bioactive compounds using
photosynthesis. Microalgae contain a variety of high value-added natural pigments such as …
photosynthesis. Microalgae contain a variety of high value-added natural pigments such as …
Next-generation breeding strategies for climate-ready crops
Climate change is a threat to global food security due to the reduction of crop productivity
around the globe. Food security is a matter of concern for stakeholders and policymakers as …
around the globe. Food security is a matter of concern for stakeholders and policymakers as …
Plant base editing and prime editing: the current status and future perspectives
J Li, C Zhang, Y He, S Li, L Yan, Y Li… - Journal of Integrative …, 2023 - Wiley Online Library
Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …
in a predefined manner by gene editing technologies is highly desirable in crop …
Breeding custom‐designed crops for improved drought adaptation
The current pace of crop improvement is inadequate to feed the burgeoning human
population by 2050. Higher, more stable, and sustainable crop production is required …
population by 2050. Higher, more stable, and sustainable crop production is required …
Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system
Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …
sourced from a human pathogen, is the most widely used. Here, through in silico data …