It has long been appreciated that the Gram-negative outer membrane acts as a permeability
barrier, but recent studies have uncovered a more expansive and versatile role for the outer …

Abstract The Gram-positive bacterium Bacillus subtilis can initiate the process of sporulation
under conditions of nutrient limitation. Here, we review some of the last 5 years of work in …

The peptidoglycan cell wall and the actin-like MreB cytoskeleton are major determinants of
cell shape in rod-shaped bacteria. The prevailing model postulates that helical, membrane …

We present a single molecule method for counting proteins within a diffraction-limited area
when using photoactivated localization microscopy. The intrinsic blinking of photoactivatable …

Highlights•The discovery of the FtsZ ring by Bi and Lutkenhaus in 1991 was a defining
moment in the field of bacterial cell division.•The past 25 years of research have significantly …

Ancestral homologues of the major eukaryotic cytoskeletal families, tubulin and actin, play
critical roles in cytokinesis of bacterial cells. FtsZ is the ancestral homologue of tubulin and …

Endospore formation has been a rich field of research for more than a century, and has
benefited from the powerful genetic tools available in Bacillus subtilis. In this review, we …

Much of what we know about cytokinesis in bacteria has come from studies with Escherichia
coli, and efforts to comprehensively understand this fundamental process in this organism …

Endospore formation in Bacillus subtilis provides an ideal model system for studying
development in bacteria. Sporulation studies have contributed a wealth of information about …

Fengycin is a lipopeptide with broad-spectrum antifungal activity. However, its low yield
limits its commercial application. Therefore, we iteratively edited multiple target genes …