Enzymatic Oxy‐and Amino‐Functionalization in Biocatalytic Cascade Synthesis: Recent Advances and Future Perspectives

E Grandi, F Feyza Özgen, S Schmidt… - Angewandte …, 2023 - Wiley Online Library
Biocatalytic cascades are a powerful tool for building complex molecules containing oxygen
and nitrogen functionalities. Moreover, the combination of multiple enzymes in one pot offers …

Chemoenzymatic Synthesis of Fluorinated L-α-Amino Acids

X Jin, C Huang, C Cui, H Liu, Z Liu… - ACS Sustainable …, 2024 - ACS Publications
Fluorinated amino acids play a crucial role as fundamental components in drug synthesis
owing to their favorable attributes that enhance the pharmacokinetic properties of drugs …

Direct asymmetric synthesis of β-branched aromatic α-amino acids using engineered phenylalanine ammonia lyases

C Sun, G Lu, B Chen, G Li, Y Wu, Y Brack, D Yi… - Nature …, 2024 - nature.com
Abstract β-Branched aromatic α-amino acids are valuable building blocks in natural
products and pharmaceutically active compounds. However, their chemical or enzymatic …

Engineered Zea mays phenylalanine ammonia-lyase for improve the catalytic efficiency of biosynthesis trans-cinnamic acid and p-coumaric acid

J Zheng, R Sun, D Wu, P Chen, P Zheng - Enzyme and Microbial …, 2024 - Elsevier
Phenylalanine ammonia-lyase (PAL) plays a pivotal role in the biosynthesis of
phenylalanine. PAL from Zea mays (ZmPAL2) exhibits a bi-function of direct deamination of …

Functional and Mechanistic Dissection of Protein Glutaminase PG3 and Its Rational Engineering for Enhanced Modification of Myofibrillar Proteins

W Leng, Y Li, L Yuan, X Li, R Gao - Journal of Agricultural and …, 2024 - ACS Publications
Protein glutaminases (PG; EC= 3.5. 1.44) are enzymes known for enhancing protein
functionality. In this study, we cloned and expressed the gene chryb 3 encoding protein …

Engineering phenylalanine ammonia lyase to limit feedback inhibition by cinnamate and enhance biotransformation

S Pavale, SK Dalei, P Sokhal, B Biswas… - Biotechnology …, 2024 - Wiley Online Library
Phenylalanine ammonia‐lyase (PAL) is a crucial enzyme for various biotechnology
applications, such as producing phenols, antioxidants, and nutraceuticals. However …

Engineered, Scalable Production of Optically Pure l-Phenylalanines Using Phenylalanine Ammonia-Lyase from Arabidopsis thaliana

SD Tork, EZA Nagy, RB Tomoiagă… - The Journal of Organic …, 2022 - ACS Publications
An efficient preparative-scale synthetic procedure of l-phenylalanine derivatives has been
developed using mutant variants of phenylalanine ammonia-lyase from Arabidopsis thaliana …

Engineered Phenylalanine Ammonia‐Lyases for the Enantioselective Synthesis of Aspartic Acid Derivatives

I Buslov, S Desmons, Y Duhoo, X Hu - Angewandte Chemie, 2024 - Wiley Online Library
Biocatalytic hydroamination of alkenes is an efficient and selective method to synthesize
natural and unnatural amino acids. Phenylalanine ammonia‐lyases (PALs) have been …

Discovery and mechanistic analysis of a novel source protein glutaminase PG5 and its potential application

W Leng, Y Li, X Liang, X Li, R Gao - Food Chemistry, 2024 - Elsevier
In this study, we successfully obtained a novel source protein glutaminase PG5 with specific
activity of 10.4 U/mg, good tolerance and broad substrate profile through big data retrieval …

Stereoselective Synthesis of Aliphatic α‐Amino Acids by Chemo‐Enzymatic Hydroamination‐Hydrodesulfurisation

C Nobbio, WR Birmingham, E Brenna… - Advanced Synthesis …, 2025 - Wiley Online Library
Phenylalanine ammonia lyase (PAL) enzymes have been extensively exploited to produce
differently substituted arylalanine analogues, but their use in the stereoselective synthesis of …