DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds
bidirectionally, resulting in two replication forks that travel in opposite directions from the …

Initiation of DNA replication is required for cell viability and passage of genetic information to
the next generation. Studies in Escherichia coli and Bacillus subtilis have established A …

Solid-state NMR spectroscopy has developed into a useful method for protein structure
determination. In contrast to diffraction methods, NMR spectroscopy does not rely on long …

Cellular replication forks are powered by ring-shaped, hexameric helicases that encircle and
unwind DNA. To better understand the molecular mechanisms and control of these …

Replicative helicases are essential ATPases that unwind DNA to initiate chromosomal
replication. While bacterial replicative DnaB helicases are hexameric, Helicobacter pylori …

Motor proteins are involved in a variety of cellular processes. Their main purpose is to
convert the chemical energy released during adenosine triphosphate (ATP) hydrolysis into …

Hexameric DnaB helicases are often loaded at DNA replication forks by interacting with the
initiator protein DnaA and/or a helicase loader (DnaC in Escherichia coli). These loaders are …

Segmental isotope labelling enables the NMR study of an individual domain within a
multidomain protein, but still in the context of the entire full-length protein. Compared to the …

Assembly of bacterial ring-shaped hexameric replicative helicases on single-stranded (ss)
DNA requires specialized loading factors. However, mechanisms implemented by these …

The β‐clamp is the processivity‐promoting factor for most of the enzymes in prokaryotic DNA
replication; hence, it is a crucial drug target. In the present study, we investigated the β …