Recent successes illustrate the role of mass spectrometry-based proteomics as an
indispensable tool for molecular and cellular biology and for the emerging field of systems …

The targets of in vivo studies of the ribosomal assembly process are mainly the events of
rRNA processing, whereas in vitro studies (total reconstitution) focus on principls of the …

The ubiquitin (Ub)-proteasome system includes a large family of deubiquitinating enzymes
(DUBs). Many members are assigned to this enzyme class by sequence similarity but …

The conserved histone variant H2A. Z functions in euchromatin to antagonize the spread of
heterochromatin. The mechanism by which histone H2A is replaced by H2A. Z in the …

Kinetics of in-gel digestion of proteins by modified and native trypsins was studied by MALDI
TOF mass spectrometry using 18O-labeled peptides as internal standards. The effect of the …

The purification of protein complexes can be accomplished by different types of affinity
chromatography. In a typical immunoaffinity experiment, protein complexes are captured …

Tandem affinity purification (TAP) strategies constitute an efficient approach for protein
complex purification from many different organisms. However, the application of such …

Affinity chromatography is a powerful protein separation method that is based on the specific
interaction between immobilized ligands and target proteins. Peptides can also be …

Defining protein complexes is critical to virtually all aspects of cell biology because many
cellular processes are regulated by stable protein complexes, and their identification often …

The eukaryotic cytosolic chaperonin containing TCP‐1 (CCT) has an important function in
maintaining cellular homoeostasis by assisting the folding of many proteins, including the …