aprE 基因表达的分子生物学和微量热法分析
李美菊， 陈向东， 刘国生， 杨洋， 王小娟， 耿晶， 梅运军… - 化学学报, 2005 - sioc-journal.cn
摘要利用分子生物学方法(SDS-PAGE 和酶活检测法) 未检测到所克隆的aprE
基因在大肠杆菌中的表达产物(碱性蛋白酶), 而微量热法检测结果发现: 重组菌株的生长代谢产热 …
基因在大肠杆菌中的表达产物(碱性蛋白酶), 而微量热法检测结果发现: 重组菌株的生长代谢产热 …
极端嗜盐古生菌启动子序列缺失突变的微量热研究
朱建裕， 刘义， 胡岳华， 曾驰， 张立侠， 崔长征， 黄玉屏… - 化学学报, 2006 - sioc-journal.cn
摘要用微量热方法和DNA 缺失突变技术研究了来源于极端嗜盐古生菌R1
上的一个推测的启动子片段(RM10) 在大肠杆菌中的启动子功能. 启动子片段融合到质粒 …
上的一个推测的启动子片段(RM10) 在大肠杆菌中的启动子功能. 启动子片段融合到质粒 …
Microcalorimetric investigation on the growth model and the protein yield of Bacillus thuringiensis
L Xiaoyan, L Yi, L Peng, Q Songsheng… - Journal of biochemical and …, 2004 - Elsevier
A novel microcalorimetric technique based on the bacterial heat output was applied to
evaluate the special growth model, the protein expression and the generation time of …
evaluate the special growth model, the protein expression and the generation time of …
Microcalorimetric study on the transcription start site mutagenesis
Y Yang, Y Liu, J Zhu, P Shen - Journal of thermal analysis and …, 2004 - akjournals.com
The microcalorimetric method and DNA site-directed mutagenesis technique were used
together to study the effect of transcription start site mutagenesis on RM07 promoter activity …
together to study the effect of transcription start site mutagenesis on RM07 promoter activity …
Analysis of aprE gene expression employing microcalorimetric technique and molecular biological method
MJ LI, XD CHEN, GS LIU, Y YANG, XJ WANG… - Acta Chimica …, 2005 - sioc-journal.cn
The expression product of the cloned aprEin E. coli failed to be detected by general
molecular biological methods including SDS-PAGE and protease activity determination …
molecular biological methods including SDS-PAGE and protease activity determination …
A microcalorimetric method for studying Halobacterium halobium
YP Huang, Y Liu, P Shen, SS Qu - Journal of thermal analysis and …, 2003 - Springer
The application of a microcalorimetric method to the study of extremophiles is described
briefly. Using the LKB 2277 Bioactivity Monitor, the growth thermogenic curves of three …
briefly. Using the LKB 2277 Bioactivity Monitor, the growth thermogenic curves of three …
Study on the nucleotide mutation in-35 box sequence of gene promoter by means of microcalorimetric method
P Shen - Journal of thermal analysis and calorimetry, 2005 - Springer
The important thermochemistry research method-microcalorimetry was successfully applied
to study the effect of different nucleotide mutations in-35 box sequence on the gene …
to study the effect of different nucleotide mutations in-35 box sequence on the gene …
Microcalorimetric research on recombinant Escherichia coli with high production of polyhydroxyalkanoates (PHAs)
L Ruan, Y Wang, L Wai, Y Hoi-Fu - Journal of thermal analysis and …, 2007 - Springer
The thermogenic curves of metabolism of the four strains of Escherichia coli
pUC19cab/JM109, pUC19cab/XL-IBlue, JM109 and XL-IBlue were determined using the …
pUC19cab/JM109, pUC19cab/XL-IBlue, JM109 and XL-IBlue were determined using the …
Microcalorimetric investigation of the growth of the engineering BACILLUS THURINGIENSIS with different plasmid numbers and various promoters
L Xiaoyan, L Yi, S Ming, L Peng, Z Juncheng… - Journal of thermal …, 2005 - Springer
A microcalorimetric technique based on the bacterial heat output was applied to evaluate
the influence of plasmid copy number and various promoters on the green fluorescent …
the influence of plasmid copy number and various promoters on the green fluorescent …