Contamination in low microbial biomass microbiome studies: issues and recommendations

R Eisenhofer, JJ Minich, C Marotz, A Cooper… - Trends in …, 2019 - cell.com
Next-generation sequencing approaches in microbiome research have allowed surveys of
microbial communities, their genomes, and their functions with higher sensitivity than ever …

Identifying biases and their potential solutions in human microbiome studies

JT Nearing, AM Comeau, MGI Langille - Microbiome, 2021 - Springer
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …

Aridity-driven shift in biodiversity–soil multifunctionality relationships

W Hu, J Ran, L Dong, Q Du, M Ji, S Yao, Y Sun… - Nature …, 2021 - nature.com
Relationships between biodiversity and multiple ecosystem functions (that is, ecosystem
multifunctionality) are context-dependent. Both plant and soil microbial diversity have been …

Late Quaternary dynamics of Arctic biota from ancient environmental genomics

Y Wang, MW Pedersen, IG Alsos, B De Sanctis… - Nature, 2021 - nature.com
During the last glacial–interglacial cycle, Arctic biotas experienced substantial climatic
changes, yet the nature, extent and rate of their responses are not fully understood …

Uses and misuses of environmental DNA in biodiversity science and conservation

ME Cristescu, PDN Hebert - Annual Review of Ecology …, 2018 - annualreviews.org
The study of environmental DNA (eDNA) has the potential to revolutionize biodiversity
science and conservation action by enabling the census of species on a global scale in near …

Environmental DNA for wildlife biology and biodiversity monitoring

K Bohmann, A Evans, MTP Gilbert, GR Carvalho… - Trends in ecology & …, 2014 - cell.com
Extraction and identification of DNA from an environmental sample has proven noteworthy
recently in detecting and monitoring not only common species, but also those that are …

The detection of aquatic animal species using environmental DNA–a review of eDNA as a survey tool in ecology

HC Rees, BC Maddison, DJ Middleditch… - Journal of applied …, 2014 - Wiley Online Library
Knowledge of species distribution is critical to ecological management and conservation
biology. Effective management requires the detection of populations, which can sometimes …

Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

SJ Salter, MJ Cox, EM Turek, ST Calus, WO Cookson… - BMC biology, 2014 - Springer
Background The study of microbial communities has been revolutionised in recent years by
the widespread adoption of culture independent analytical techniques such as 16S rRNA …

Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples

A Glassing, SE Dowd, S Galandiuk, B Davis… - Gut pathogens, 2016 - Springer
Background The advent and use of highly sensitive molecular biology techniques to explore
the microbiota and microbiome in environmental and tissue samples have detected the …

Towards next‐generation biodiversity assessment using DNA metabarcoding

P Taberlet, E Coissac, F Pompanon… - Molecular …, 2012 - Wiley Online Library
Virtually all empirical ecological studies require species identification during data collection.
DNA metabarcoding refers to the automated identification of multiple species from a single …