Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Specialized epitope tags are widely used for detecting, manipulating or purifying proteins,
but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed …

The endoplasmic reticulum (ER) undergoes continuous remodelling via a selective
autophagy pathway, known as ER-phagy. ER-phagy receptors have a central role in this …

Super-resolution techniques have begun to transform biological and biomedical research by
allowing researchers to observe structures well below the classic diffraction limit of light …

DNA-PAINT's imaging speed has recently been significantly enhanced by optimized
sequence design and buffer conditions. However, this implementation has not reached an …

The specific and covalent labeling of the protein HaloTag with fluorescent probes in living
cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the …

Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations
have been defined not only by abundant tetraspanins (eg, CD9, CD63 and CD81) but also …

Advances in super-resolution microscopy have demonstrated single-molecule localization
precisions of a few nanometers. However, translation of such high localization precisions …

DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-
resolution fluorescence microscopy technique that achieves single-molecule 'blinking'by …